Спутник ФКИ. E1P. [Редактировать]

Спутник E1P (Explorer-1 [PRIME]) разработки университета штата Монтана повторит с помощью современных технологий эксперимент, который был поставлен с помощью первого в истории США спутника Explorer 1 в 1958 году. Последний обнаружил существование поясов ван Аллена - областей, в которых накапливаются "пойманные" магнитосферой Земли высокоэнергичные заряженные частицы.

Дополнительная классификация

#Наименования
1Тип оператора(владельца) - государственный
2Страна оператор(владелец) - США
3Страна производитель - США

Информация об удачном запуске

#ХарактеристикаЗначение
1Космодром База Ванденберг
2Дата пуска2011-10-28 at 09:48:00 UTC
3Полезная нагрузка 1xNPOESS Preparatory Project
4Полезная нагрузка 1xDICE 1
5Полезная нагрузка 1xDICE 2
6Полезная нагрузка 1xRAX-2
7Полезная нагрузка 1xAubieSat -1
8Полезная нагрузка 1xE1P
9Полезная нагрузка 1xMCubed-2
10Ракета-носитель 1xДельта 2 7920

Найдено 102 документов по запросу «E1P». [Перейти к поиску]


Дата загрузки: 2017-11-08
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0.11/5
... of the phosporylated pyruvate dehydrogenase (E1p) component of the human pyruvate... diphosphate (ThDP)-dependent pyruvate dehydrogenase (E1p) component, the FAD-containing dihydrolipoamide... (5) FADH2 + NAD + /FAD + NADH + H + (6) The E1p component catalyzes the ThDP-mediated... Structure Crystal Structures of Phospho-E1p Component Figure 1. Overall Structure of...). Phosphorylation of the heterotetrameric (a2b2) E1p component by PDK isoforms occurs... exhibits different site specificity. Using E1p mutants with single functional phosphorylation... deciphering crystal structures of a phophorylated E1p variant, in which sites 2 and...-E1p Component Figure 2. Time Course for Phosphorylation of E1p Variants by PDK4 E1p variants S1-E1p and S2-E1p contain... mutated to alanine. S1-E1p (C) and S2-E1p (,) were phosphorylated by PDK4... to percent phosphorylation. Each variant E1p heterotetramer has two phosphorylatable serine...) allowed efficient phosphorylation of the E1p variant without geometric constraints imposed... E1p preparations, E1p variants containing only phosphorylation site 1 (S1-E1p) or site 2 (S2-E1p..., when the S1-E1p or the S2-E1p single-site variant and... al., 2008). Free S1-E1p or S2-E1p was incubated for up... 1 and 2 with the S1-E1p and S2-E1p mutants, respectively, was achieved...-up fully phosphorylated S1-E1p (phospho-S1-E1p) was used in the... crystallographic studies. Phosphorylation of S1-E1p Abolishes Activities for Reductive Acetylation... studies of wild-type E1p, S1-E1p, and phosphoS1-E1p were carried out... overall reaction (reaction 1), E1p-catalyzed decarboxylation (reaction 2), and E1p-catalyzed reductive acetylation... Phospho-E1p Component Table 1. Kinetic Parameters for Wild-Type E1p, S1-E1p, and Phospho-S1-E1p Assays kcat (sÀ1) Km... (sÀ1mMÀ1) Overall WT E1p 21 ± 1 41 ± 4 0.51 Y89F-a E1p 19 ± 3 78 ± 13 0.24 S1-E1p 18 ± 2 58 ± 5 0.31 Phospho-S1-E1p NM NM - DCPIP WT E1p Y89F-a E1p 0.26 ± 0.02 8.1 ± 0.5 0.032 2.7 ± 0.05 31 ± 5 0.087 S1-E1p 0.31... E1p 0.030 ± 0.002 Y89F-a E1p 0.028 ± 0.002 S1-E1p 0.028 ± 0.001 - - Phospho-S1-E1p NM - - Phosphorylation studies on S1-E1p... values for wild-type E1p and S1-E1p by 19% and 32... of the wild-type E1p (8.1 mM) and S1-E1p (9.5 mM). This finding... substrate pyruvate. In parallel experiments, E1p-catalyzed reductive acetylation (reaction 3) was... L2 domain. Wild-type E1p and S1-E1p show essentially identical kcat... sÀ1 (Table 1). Notably, phosphorylation of S1-E1p reduces reductive acetylation activity to... methylacetylphosphonate (MAP) to the S264E-a E1p variant, compared with wild-type...-E1p remains unknown. S1-E1p Structure Is Unperturbed Compared with Wild-Type E1p... the human E1p heterotetramer: (1) wild-type E1p (containing Mn-ThDP), (2) S1-E1p (containing... two previously determined human E1p structures: wild-type E1p (Mg-ThDP; PDB... saturating ThDP concentrations, this mutant E1p shows essentially wild-type activity... overall reaction as well as E1p-catalyzed decaroxylation and reductive acetylation (Table 1). The Y89F-a E1p structure shows no electron density... Structure Crystal Structures of Phospho-E1p Component Table 2. Data Collection and... E1p (Mn-ThDP) S1-E1p (Mg-ThDP) Phospho-S1-E1p (apo) Phospho-S1-E1p (Mn-ThDP) Y89F-a E1p... to Wild-Type E1p, S1-E1p, and Phospho-S1-E1p in the Presence... (%) Wild-type E1p 0.11 ± 0.01 76.1 ± 2.1 Y89F-a E1p 50.2 ± 4.6 67.5 ± 2.3 S1-E1p 0.29 ± 0.02 68.4 ± 2.5 Phospho-S1-E1p 13... in the nonphosphorylated wild-type E1p structure. This conformation potentially imposes... Crystal Structures of Phospho-E1p Component wild-type E1p-a subunit (Figure 4E... of wild-type E1p, S1-E1p, and phospho-S1-E1p for cofactor ThDP... phosphorylation site 1 of E1p (S264E-a and S264Q-a E1p variants) did not alter... Structure Crystal Structures of Phospho-E1p Component Figure 5. Extensive Interactions between... Binding to the Phosphorylated S1-E1p Protein Isothermal titration calorimetry (ITC... the ordered conformations in the E1p component of the Bacillus stearothermophilius... to E1p Determined by Isothermal Titration Calorimetry Wild-type E1p, S1-E1p, or phospho-S1-E1p protein (35 mM... 7.0 software. For wild-type E1p and S1-E1p, the dissociation constants Kd... 1 Phosphorylation in E1p (A) Reaction scheme for nonphosphorylated E1p. The E1p active site is... panel). (B) Inactivation mechanism for phosphorylated E1p. When Ph-loop A is disordered...). Under saturating ThDP conditions, the E1p-catalyzed decarboxylation of pyruvate proceeds.... Phosphorylation of E1p Phosphorylation of S1-E1p or S2-E1p was carried out...-type E1p), 3EXF (S1-E1p), 3EXG (apo phospho-S1-E1p), 3EXH (phospho-S1-E1p with Mn-ThDP), and 3EXI (Y89F-a E1p... Structure Crystal Structures of Phospho-E1p Component Harris, R.A., Bowker-Kinley, M.M., Huang...



Дата загрузки: 2017-11-08
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0.48/5
... two principal reactive states, E1P and E2P. E1P is formed reversibly from... observed decay kinetics. We observed E1P or E2P selectively by adding... ADP respectively. Kϩ dephosphorylates E2P leaving E1P as observable species; ADP dephosphorylates... 1Abbreviations used in this paper: E1P, E2P, reactive states (forms, conformations... the phosphointermediate are named E1P and E2P. E1P donates its phosphate group... (Jørgensen, 1992; Horisberger, 1994): Na 3 E1P + Na 2 E2P + Na . (2) ClϪ, We... of transphosphorylation of E1P: ATP ⋅ E1 ADP ⋅ E1P; K int [ ADP ⋅ E1P ] = --------------------------------[ ATP ⋅ E1... follows: Na 3 E1 Na Na 3 E1P + Na 2 E1P + + Na 2 E2P + Na . (4) We... the partition between the phosphointermediates, E1P and E2P, according to a Hofmeister... E2P whereas chaotropic anions favor E1P (Post and Suzuki, 1991; Klodos... seen much larger differences in E1P-E2P partition between different preparations... were estimated as follows. Kϩ-resistant E1P was isolated by including Kϩ in... absence of ADP. ADP dephosphorylates E1P immediately (on the time scale... Post Interconversion Rate Constant E2P E1P k21 Interconversion Rate Constant k10... salt increased the amount of E1P. In some experiments arginineϩ, N-methylglucamine... phosphointermediate as K-resistant E1P. For ADP chases of E1P the concentration of... The equilibrium constant between phosphointermediates E1P and E2P was estimated from...) were those of E1P and E2P together. Kϩ-resistant E1P and ADP-resistant... rate constants of interconversion between E1P and E2P. The 2-pool model... phosphointermediates following chases that left E1P, E2P or both as the... formation the phosphointermediate components decay. E1P always decreases progressively in amount... rate constants. Observed E1P is the sum of E1P in the rapid... technique formation of labeled phosphointermediate (E1P plus E2P) is terminated by..., Kϩ or ADP, to estimate K-resistant E1P or ADP-resistant E2P respectively. Thus interconversion between E1P and E2P proceeds for an... that partition the phosphointermediate between E1P and E2P (Post and Suzuki... is formed later from E1P is dephosphorylated. E1P decays all by itself... all E2P is dephosphorylated; only E1P remains. And during its subsequent... partition of the phosphointermediate between E1P and E2P to reach a steady... in materials and methods. K-resistant E1P was estimated by addition of... concentration of 6 mM KCl (᭿ or ᮀ, E1P) at zero time (᭿) as a standard... 0.008 Conversion of E2P to E1P, k21 /s 0.038 0.005 2.0 0.35 Equilibrium..., s Parameter Units 6 30 P*, % Hydrolysis of E1P, k10 /s 0.043 0.049 Ͼ 10 Hydrolysis... 0.036 0.1 Conversion of E2P to E1P, k21 /s 0.021 0.012 2.3 2.5 3.0 Equilibrium constant... with a precursor–product relationship between E1P and E2P before the chases... in Fig. 1: (᭺) blank chase, (᭿, ᮀ) K-resistant E1P, (᭡, ᭝) ADP-resistant E2P, (᭿, ᭡) standard, or... constant of interconversion. (Hydrolysis of E1P increases the ratio of dephospho..., Naϩ increased the proportion of E1P by stimulating (weakly) hydrolysis of... hydrolysis of E1P. Thus the difference in hydrolysis of E1P may depend... much more effective in stabilizing E1P than acetate was and Hofmeister... all concentrations of Naϩ. late E1P significantly. (In this experiment the... shifts the interconversion equilibrium between E1P and E2P in favor of... small amount of E1P. The initial amount of E1P was 36% in... a Hofmeister series. Chaotropic ions favored E1P and cosmotropic anions favored E2P... Naϩ on Transphosphorylation from E1P to ADP E1P donates its phosphate group... [␥-32P]ATP under conditions where E1P was predominant and relatively stable... the following: KS ADP + E1P K int ADP ⋅ E1P k off ATP ⋅ E1 → E1... irreversible dissociation, koff. KS ϭ [ADP][E1P]/[ADPиE1P]. Kint ϭ [ADPиE1P]/[ATPиE1]. koff... model predicts a biphasic dephosphorylation of E1P on a semilogarithmic plot. An instantaneous... radioactive intermediate. To isolate K-resistant E1P we included 6 mM KCl in... ADP with the phosphointermediate: ADP ϩ E1P ADPиE1P ATPиE1 whereas the slow... of Naϩ on Transphosphorylation from E1P to ADP. *NMG, N-methylglucamine. Et3N... other was an equilated intermediate, E1P librium of transphosphorylation, ATPиE1 ADPиE1P... with the dephosphoenzyme than to E1P. E1P and E2P are thought to... (Figs. 1–3). The diagnostic reactions are: E1P ϩ ADP → E1 ϩ ATP and E2P.... Naϩ increased the proportion of E1P in the phosphointermediate but did... it also stimulated hydrolysis of E1P (Fig. 4 and experiments not shown... of hydrolysis of E1P. Stimulation of hydrolysis of E1P cannot be a K-like... between ATPиE1 and ADPиE1P. When E1P was stabilized by a Hofmeister effect... reaction: ATP ⋅ E1 ⋅ Na 3 + ADP ⋅ E1P ⋅ Na 2 + Na . (7) This reaction implies... Naϩ can be released from E1P before E1P changes to E2P. Release... ⋅ E1 § cyt ⋅ Na 3 ADP ⋅ E1P § ( Na 3 ) + . ADP ⋅ E1P § ( Na 2 ) + Na ext (8) Presumably... modifications also inhibit conversion of E1P to E2P. However, inhibition of.... We needed comparable amounts of E1P and E2P to estimate interconversion... submodel consists of two phosphointermediates, E1P and E2P. Binding of ligands...



Дата загрузки: 2017-11-08
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0.25/5
... coliof Genes Encoding theEla and E1P Subunits of the Pyruvate Dehydrogenase... CB2lQW, United Kingdom The E l a and E1P subunits of the pyruvate decarbox... al., 1989). Althoughno 2-oxoacid dehydrogenase E1P subunit existed mainly (95%) in... compose the E2component combinant E l a and E1P subunits in vitro generated a have... theEla (Davie e t al., 1992) and E1P (Wynn et al, 1992a) chainsof... overexpressed in E. coli. The recombinant E1P subunit, but not the Ela... encoding the B. stearothermophilus Ela and E1P subunits separately, the purification of... on nitrogen. For the recombinant E1P subunit, after the DE52 column... onto KBstEla of 0.3-0.5 M NaCl recombinant E1P chain eluted with a linear gradient... (0.25 ml) containing the recombinant E1P subunitwere pooled, dialyzedovernightagainst buffer C, and... from the purified Ela and E1P subunits was analyzed by measuring... the pure recombinant Ela and E1P subunits in molar ratios of... form, but 25% of the E1P subunit was found in the... for the overexpression of the E1P chain of the mammalian branched... cells expressing the Ela and E1P Construction of Expression Vectors pKBstEla... together. An mophilus Ela and E1P genes from the same promoter...) association of the Ela and E1P subunits was indicated by chrosince... Ela gene upstream of the E1P gene. How- followed by a PorosTMQ/Mcolumn (Fig. 3). Excess E1P subunit ever, all efforts to... similar to that of the E1P subunit when it was pugenes... expression of the Ela and E1P genes was >95% pure as... pKBstElb for the matureEla and E1P subunits, respectively, calibrated SuperdexTM200HR gel.... protein synthesis upstream of the E1P gene was replaced with This... from the amino sequences of E1P gene from plasmid pKBstEl.l, in... the B. stearotherlowed by the complete E1P gene wascloned into theexpression mophilus... expression (see below Ela and E1P genes is sufficient. Quaternary Structure... the poor expression of the E1P gene from plasmid pKBstEl.l werenot investigated vidual Ela and E1P subunits generated by expression of the Ela and E1P genes from plasmids pKBstEla and... cellular levels of Ela and E1P proteins thatreached -35 and the pureB. stearothermophilus Ela and E1P subunits obtained 50%, respectively, of... their quaternary structure, theEla and E1P subprocessed in vivo to remove... in the text. The E l a and E1p subunits were then purified from... other hand,>95% of the E1P subunit eluted ina peak with... quantity of each Ela or E1P chain, were compared with the... extract (Fig. 5). When the recombinant E1P subunit was mixed with a n excess... of recombinant E l based on its E1P subunit content. This suggests that... chains bound to the recombinant E1P chains to create likewise a fully... anequimolar mixture of Ela and E1P chains, only 92%of the... 8% of the recombinant Ela and E1P chains present failed to assemble... the remaining noncomplexed Ela and E1P chains eluted separately(Fig. 6). Molar... E l a and E l P subunits. The purifiedE l a and E1p subunits were mixed a t different molar... molar quantityof the Ela (0) or E1p (0) chain present in the assay... the El(azpz)enzyme. 0, adding E1p to a fixed amount (300 pmol... genes encoding the Ela and E1P subunits of the E l component from... E l a and ElP subunits. a,the E l n and E1P subunits were mixed at a molar... folding of the individual E l a and E1P subunits, which might explain the... and overexpression of the E l a and E1P genes, this and other aspects...



Дата загрузки: 2017-01-30
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0.09/5
.../1y ,aqV\-\ `\/*x1 L5 SY\O e1p 6B8MD 5; Ob`1bnSfE a]`*,1/WS,\abn...



Дата загрузки: 2017-01-30
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0.06/5
...*yZ\ b`d/Zm1 ,`1Y1Z*x L6 SY\O e1p 7B5MD Ä1a]`*,1/WS,\abn S d-Z1b1ZS1... S /*`SW `*/\abn /`d-SY L5 SY\O e1p 5B55C :B59MD /Z*3/m \Z ]`Sj1W Z* a,*/n+d P * ob\ a\+mbS1E...



Дата загрузки: 2017-02-23
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0.12/5
... double complex F (I •,• ) satisfy the relations E1p,q = Rq F (K p ) and E2p,q = Rp F (H q (K • )) Moreover... sequence. We have E1p,q = H q (Lp,• ) in other words E1p,q = H q (F (I p,• )) = Rq F (K p ) as desired... use that the maps dp,q : E1p,q → E1p+1,q are the 1 q p q p+1 p p+1 maps R F (K ) → R F (K ) induced by... second spectral sequence. We have E1p,q = H q (L•,p ) = H q (F (I •,p )). Note that the complex I •,p is... is true after applying F . Hence E1p,q = F (H q (I •,p )) = F (HIq (I •,p )). The differentials on this... dr of bidegree (r, −r + 1) and with E1p,q = Rp+q T (grp (K • )) Moreover, this spectral... Homology, Lemma 21.2 we have E1p,q = H p+q (grp (T (I • ))). By Equation (26.13.3) we have E1p,q = H p+q (T (grp (I • ))), and by definition of... (I • ) is an injective resolution. Hence E1p,q = Rp+q T (grp (K • )). On the other... a spectral sequence with E1 term E1p,q = Rp+q T (K p [−p]) = Rq T (K p ) as in the... . Observe moreover that the differentials E1p,q → E1p+1,q agree with the differentials in... a spectral sequence with E1 term E1p,q = Rp+q T (H −p (K • )[p]) = R2p+q T (H −p (K • )) = E2i,j DERIVED CATEGORIES...



Дата загрузки: 2017-11-08
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0.42/5
... Pumping and Accumulation in the E1P Conformational State of a Plant Plasma... an aspartyl phosphate enzyme intermediate E1P is 1 The abbreviations used are... through the E1P-E2P transition. 39168 Isolation of the E1P Conformational State... change is associated with the E1P to E2P transition. During transition... transport by P-type ATPases. The E1P Ϫ E2P conformational change is attracting... mutation caused a block in the E1P to E2P conformational change. We... be transported. Isolation of the E1P Conformational State of a P-type ATPase... a standard. 39170 Isolation of the E1P Conformational State of a P-type ATPase... in the conformational change from E1P to E2P during which the... for by a slowing of the E1P to E2P conformational change; as a result, the ATPase accumulates in E1P, which has a low affinity for...⌬73D684N, respectively. Isolation of the E1P Conformational State of a P-type ATPase... could be blocked in the E1P-E2P transition. The stability of... this nucleotide (Fig. 7B). Since E1P, in contrast to E2P, is... enzyme is blocked in the E1P state. DISCUSSION FIG. 6. Trypsination pattern...-type 39172 Isolation of the E1P Conformational State of a P-type ATPase... in its catalytic cycle toward E1P. However, direct evidence for proton... mutant enzyme accumulates in the E1P conformational state. The corresponding residue... the conformational change between the E1P and E2P forms. It has... Mutant Is Blocked in the E1P-E2P Conformational Transition—The Asp684... us that it was the E1P form of the mutant enzyme..., which interacts specifically with the E1P form of P-type ATPases; (ii... Hϩ-ATPase would likely involve direct E1P hydrolysis. This might explain the... that a car- Isolation of the E1P Conformational State of a P-type ATPase...



Дата загрузки: 2017-01-30
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0.18/5
... PNOZ e1p 4 4 4 D Betriebsanleitung GB Operating instructions F Manuel d'utilisation Sicherheitsschaltgerät PNOZ e1p Safety Relay PNOZ e1p Bloc logique de sécurité PNOZ e1p Das Sicherheitsschaltgerät PNOZ e1p dient... PNOZelog. The safety relay PNOZ e1p is used for the safety... bloc logique de sécurité PNOZ e1p assure de façon sûre... votre sécurité Das Sicherheitsschaltgerät PNOZ e1p erfüllt alle notwendigen Bedingungen... hervorgehoben. The safety relay PNOZ e1p meets all the necessary conditions... bloc logique de sécurité PNOZ e1p satisfait à toutes les conditions nécessaires.... Das Verknüpfen von PNOPZ e1p ist erst ab Version 3.0 zul... a PLC or display. The PNOZ e1p can only be linked from... afficheur. Le couplage de PNOZ e1p n’est permis qu’à partir de... A1 Y4 S11 S34 PNOZ e1p A2 S12 S22 Y32 14... A1 Y4 S11 S34 PNOZ e1p A2 S12 S22 Y32 14...éristiques Typ/ Type/ Type PNOZ e1p PNOZ e1p C D Merkmale/ Features/ Caractéristiques...



Дата загрузки: 2017-01-30
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0.14/5
... ступени паровой турбины (ПТ), кг/с; C E1P-энтальпия сухого насыщенного пара перед...+T0 R1=R(T1K) R2=R(T2K) E1P=E(T1K)+R1 C1=ALOG(T1K...+CP1(T1K)*C1)*T2K E2D=E1P-(E1P-E2S)*EFPT X2=(E(T1K)*EFR...=S(T2K)+R2/T2K SSM2=((S1P+(E1P-E2S)*(1.-EFPT)/T2K)*X1 & +(1.-X1...))*TKK PT=GS*EFPT*(X1*(E1P-E2S)+XSM*(ESM2-EKS)) P2... EP5M=EP5 ESM1=E(T1K)*(1.-X1)+E1P*X1 SSM1=S(T1K)*(1.-X1)+S1P... ступени паровой турбины(ПТ), кг/с; E1P-энтальпия сухого насыщенного пара перед...+T0 R1=R(T1K) R2=R(T2K) E1P=E(T1K)+R1 C1=ALOG(T1K...+CP1(T1K)*C1)*T2K E2D=E1P-(E1P-E2S)*PET X2=CP1(T2K...=S(T2K)+R2/T2K SSM2=((S1P+(E1P-E2S)*(1.-PET)/T2K)*X1 & +(1.-X1...))*GET PT=GS*PET*(X1*(E1P-E2S)+XSM*(ESM2-EKS)) P2... EP5M=EP5 ESM1=E(T1K)*(1.-X1)+E1P*X1 312 SSM1=S(T1K)*(1.-X1...



Дата загрузки: 2017-03-09
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0.15/5
... in Figure 1.1, kp = ep cos ϖp = e1p cos α1 + e2p cos α2 = E1 V1p... V2p cos α2 (C.1) hp = ep sin ϖp = e1p sin α1 + e2p sin α2 = E1 V1p... e2p = kp2 + h2p = e21p + e22p + e1p e2p cos θ (C.3) where θ = α1 − α2 . For a two... + e22p )) + e22p )) (C.35) ∑ [ ∂F2 Hp e1p e2p ] 2 ) − e11 (e11 e22 T1... + Hp (e21p (C.36) ∑ [ ∂F2 Hp e1p e2p ] 2 ) − e12 (e11 e22 T1... e22 ( e212 e21 ∑ [ ∂F2 Hp e1p e2p ] p + e11 e222 ∂ap ∑ [ ∂F1... e22 ( e212 e22 ∑ [ ∂F2 Hp e1p e2p ] p + e12 e222 ∂ap ∑ [ ∂F1... over θ yields: ] ∑ [ ∂F1 1 2 2 (Gp + Hp (e1p + e2p )) (1 + F12 )3/2 p ∂ap ] ∑ [ ∂F1 −F 1 2 2 V˙ 12 = (Gp + Hp (e1p + e2p )) (1 + F12 )3/2 p ∂ap ] ∑ [ ∂F2 1 2 2 ˙ V21 = (Gp + Hp (e1p + e2p )) (1 + F22 )3/2 p ∂ap ] ∑ [ ∂F2 −F2 2 2 ˙ (Gp + Hp (e1p + e2p )) V22 = (1 + F22 )3/2 p ∂ap V˙ 11...