Спутник ФКИ. IKAROS. [Редактировать]

Межпланетный зонд – МКА с солнечным парусом (проверка технологии перемещения с помощью солнечного паруса и испытание тонкопленочных фотоэлектрических преобразователей). Для высокоточного определения параметров гелиоцентрической орбиты МКА, изменяющихся под действием солнечной тяги, будут проводиться измерения в режиме интерферометрии со сверх-длинной базой (VLBI). В сложенном состоянии МКА представляет собой плоский цилиндр диаметром 1,6 м и высотой 0,8 м. На боковой стороне барабана размещен сложенный парус и система его раскрытия. Для стабилизации и последующего развертывания паруса аппарат закручивается после отделения от последней ступени РН.
Верхняя сторона центрального блока («барабана») МКА покрыта традиционными СБ, которые служат основным источником энергии для бортовой аппаратуры. На каждом из лепестков солнечного паруса приклеены две полосы из элементов тонкопленочных СБ, сделанных из аморфного кремния толщиной 25 мкм. Эти СБ являются гибкой многослойной конструкцией. Они занимают всего 5% площади паруса, но способны вырабатывать мощность до 500 Вт. МКА оснащен датчиками, измеряющими электрический заряд паруса и его температуру. Мембрана паруса изготовлена из полиамидной пленки толщиной 7,5 мкм с алюминиевым напылением. Четыре трапецеидальных лепестка образуют полотнище квадратной формы с диагональю 20 м, при этом масса мембраны составляет всего 1,8 кг. Блок управления и экспериментальные исполнительные устройства для ориентации паруса. Они представляют собой полоски с жидкокристаллическими пленками, наклеенные на четыре «трапеции» паруса вдоль сторон квадрата. Эти полоски меняют отражательные характеристики при подаче напряжения, благодаря чему создается небольшой перекос тяги. МКА несет два научных прибора. Один из них размещается в «барабане» – это поляриметрический детектор гамма-всплесков GAP, созданный в Университете Канадзава. А на лепестках «паруса» размещены датчики детектора пылевых частиц ALDN, который будет исследовать распределение пыли в межпланетном пространстве.

Дополнительные наименования

#НаименованияПоиск в новостяхПоиск в документах
1Interplanetary Kite-craft Accelerated by Radiation Of the SunНайтиНайти
236577НайтиНайти

Дополнительная классификация

#Наименования
1Страна оператор(владелец) - Япония
2Страна производитель - Япония
3Тип оператора(владельца) - государственный

Технические характеристики

#ХарактеристикаЗначение
1Масса, кг310
2Мощность, Вт500

Характеристики орбиты

#ХарактеристикаЗначение
1Расчетный срок функционирования, год0.5-1
2САС~0.5
elapsed: 3, 11 months and 6 дней

Пусковые характеристики

#ХарактеристикаЗначение
1Код NSSDC2010-020E

Экономические характеристики

#ХарактеристикаДата измеренияЗначение
1Оператор Космическое агентство Японии
2Стоимость системы , млн. долл.16.5

Информация об удачном запуске

#ХарактеристикаЗначение
1Космодром Танегасима
2Дата пуска2010-05-20 at 21:58:00 UTC
3Полезная нагрузка 1xHayato
4Полезная нагрузка 1xWaseda-Sat2
5Полезная нагрузка 1xNegai
6Полезная нагрузка 1xAkatsuki
7Полезная нагрузка 1xIKAROS
8Полезная нагрузка 1xUnitec-1
9Ракета-носитель 1xH2A202

Найдено 228 документов по запросу «IKAROS». [Перейти к поиску]


Дата загрузки: 2017-04-08
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... Center, Greece: the case of “Ikaros”. 238 *Nichols, W. J., J. A. Seminoff, C. Lockwood and... CENTRE, GREECE - THE CASE OF “IKAROS” M. Kallonas, D. Dimopoulos and D. Margaritoulis Sea... given to the case of “Ikaros”, a juvenile loggerhead that suffered from... injury. The recovery process of Ikaros is an exciting one to... Ikaros have changed dramatically over the last months. THE CASE OF “IKAROS.... NMFS-SEFSC-415 (1998) When Ikaros first arrived at the Centre... a surface area of 4.5cm x 3.0 cm. Ikaros is a juvenile turtle, on arrival.... On admission to the Centre, Ikaros was placed in freshwater for.... Over the seven months since Ikaros has been at the Centre... as eating on his own. Ikaros has been kept in sea... of year and Ikaros’ condition. THE RECOVERY PROCESS OF IKAROS OVER THE... underneath. Swimming progress. At first, Ikaros was only at the surface... breathe. Feeding progress. At first, Ikaros was fed via a drip and... fish (from 10 October 1996). Ikaros is now able to eat... spraying with the antibiotic Oxyvet. Ikaros also receives vitamin injections every... present, the blood values of Ikaros are within the normal range... abilities of Ikaros have altered. The respiratory pattern of Ikaros has changed... low (around 7 breaths/hour). As Ikaros gained strength and became more.... WHEN WILL IKAROS BE READY FOR A RELEASE? Although Ikaros is able to... always consistent. On some days, Ikaros is floating on the surface... bottom of the pool. Sometimes Ikaros will only eat if the... Ikaros becoming too accustomed to human presence. Therefore human contact with Ikaros... the sea. The temperature of Ikaros’ pool is controlled to be... acclimated prior to release. Once Ikaros is acclimated and as soon...



Дата загрузки: 2017-04-09
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SANTA CRUZ BIOTECHNOLOGY, INC. Pegasus (1B6): sc-517137 BACKGROUND APPLICATIONS Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Pegasus, also known as zinc finger protein Ikaros 5, is a 419 amino acid transcriptional repressor. Localized to the nucleus, Pegasus binds to the 5'GNNTGTNG-3' DNA core sequence. Pegasus contains five C2H2-type zinc finger domains. The C-terminal zinc finger domains participate in homodimerization and the N-terminal domains participate in heterodimerization with Ikaros family members and DNA binding. Pegasus is expressed in many tissues including kidney, heart, skeletal muscle, brain and liver. Pegasus (1B6) is recommended for detection of Pegasus of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)] and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). Suitable for use as control antibody for Pegasus siRNA (h): sc-90477, Pegasus siRNA (m): sc-146200, Pegasus shRNA Plasmid (h): sc-90477-SH, Pegasus shRNA Plasmid (m): sc-146200-SH, Pegasus shRNA (h) Lentiviral Particles: sc-90477-V and Pegasus shRNA (m) Lentiviral Particles: sc-146200-V. Molecular Weight of Pegasus: 47 kDa. Positive Controls: Pegasus transfected 293T whole cell lysate. RECOMMENDED SUPPORT REAGENTS REFERENCES 1. Perdomo, J., Holmes, M., Chong, B. and Crossley, M. 2000. Eos and Pegasus, two members of the Ikaros family of proteins with distinct DNA binding activities. J. Biol. Chem. 275: 38347-38354. 2. Nishii, K., Katayama, N., Miwa, H., Shikami, M., Usui, E., Masuya, M., Araki, H., Lorenzo, F., Ogawa, T., Kyo, T., Nasu, K., Shiku, H. and Kita, K. 2002. Non-DNA-binding Ikaros isoform gene expressed in adult B-precursor acute lymphoblastic leukemia. Leukemia 16: 1285-1292. To ensure optimal results, the following support reagents are recommended: 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). DATA 3. Online Mendelian Inheritance in Man, OMIM™. 2002. Johns Hopkins University, Baltimore, MD. MIM Number: 606238. World Wide Web URL: http://www.ncbi.nlm.nih.gov/omim/ A 4. Yoshida, T., Ng, S.Y., Zuniga-Pflucker, J.C. and Georgopoulos, K. 2006. Early hematopoietic lineage restrictions directed by Ikaros. Nat. Immunol. 7: 382-391. 5. Ng, S.Y., Yoshida, T. and Georgopoulos, K. 2007. Ikaros and chromatin regulation in early hematopoiesis. Curr. Opin. Immunol. 19: 116-122. 6. Ronni, T., Payne, K.J., Ho, S., Bradley, M.N., Dorsam, G. and Dovat, S. 2007. Human Ikaros function in activated T cells is regulated by coordinated expression of its largest isoforms. J. Biol. Chem. 282: 2538-2547. B 62 K – 150 K – 100 K – 75 K – 47.5 K – < Pegasus 50 K – < Pegasus fusion protein 37 K – 32.5 K – Pegasus (1B6): sc-517137. Western blot analysis of Pegasus expression in non-transfected (A) and Pegasus transfected (B) 293T whole cell lysates. Pegasus (1B6): sc-517137. Western blot analysis of human recombinant Pegasus fusion protein. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. CHROMOSOMAL LOCATION Genetic locus: IKZF5 (human) mapping to 10q26.13; Ikzf5 (mouse) mapping to 7 F3. RESEARCH USE SOURCE For research use only, not for use in diagnostic procedures. Pegasus (1B6) is a mouse monoclonal antibody raised against amino acids 2-100 representing partial length Pegasus of human origin. PROTOCOLS PRODUCT See our web site at www.scbt.com for detailed protocols and support products. Each vial contains 100 µg IgG2a kappa light chain in 1.0 ml PBS with < 0.1% sodium azide and 0.1% gelatin. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



Дата загрузки: 2017-02-23
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SANTA CRUZ BIOTECHNOLOGY, INC. Ikaros (E-20): sc-9861 BACKGROUND RESEARCH USE Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T-cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. For research use only, not for use in diagnostic procedures. DATA A B 105 K – 69 K – < Ikaros 43 K – 31 K – Ikaros (E-20): sc-9861. Western blot analysis of Ikaros expression in non-transfected: sc-110760 (A) and human Ikaros transfected: sc-111057 (B) 293 whole cell lysates. Ikaros (E-20): sc-9861. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization. CHROMOSOMAL LOCATION SELECT PRODUCT CITATIONS Genetic locus: IKZF1 (human) mapping to 7p12.2; Ikzf1 (mouse) mapping to 11 A1. 1. Payne, K.J., et al. 2001. Cutting edge: predominant expression of a novel Ikaros isoform in normal human hemopoiesis. J. Immunol. 167: 1867-1870. SOURCE 2. Liu, M., et al. 2004. Roles of USF, Ikaros and Sp proteins in the transcriptional regulation of the human reduced folate carrier B promoter. Biochem. J. 383: 249-257. Ikaros (E-20) is an affinity purified goat polyclonal antibody raised against a peptide mapping near the C-terminus of Ikaros of mouse origin. PRODUCT 3. Bellavia, D., et al. 2007. Notch 3 and the Notch 3-upregulated RNAbinding protein HuD regulate Ikaros alternative splicing. EMBO J. 26: 1670-1680. Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel Supershift and ChIP applications, sc-9861 X, 200 µg/0.1 ml. 4. Mullighan, C.G., et al. 2008. Bcr-Abl1 lymphoblastic leukaemia is characterized by the deletion of Ikaros. Nature 453: 110-114. Blocking peptide available for competition studies, sc-9861 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). 5. Bottardi, S., et al. 2009. Ikaros and GATA-1 combinatorial effect is required for silencing of human γ-globin genes. Mol. Cell. Biol. 29: 1526-1537. APPLICATIONS Ikaros (E-20) is recommended for detection of all Ikaros isoforms of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). Suitable for use as control antibody for Ikaros siRNA (h): sc-35640, Ikaros siRNA (m): sc-35641, Ikaros shRNA Plasmid (h): sc-35640-SH, Ikaros shRNA Plasmid (m): sc-35641-SH, Ikaros shRNA (h) Lentiviral Particles: sc-35640-V and Ikaros shRNA (m) Lentiviral Particles: sc-35641-V. 6. Thomas, R.M., et al. 2010. Ikaros silences T-bet expression and interferon-γ production during T helper 2 differentiation. J. Biol. Chem. 285: 2545-2553. 7. Alinikula, J., et al. 2010. Concerted action of Helios and Ikaros controls the expression of the inositol 5-phosphatase SHIP. Eur. J. Immunol. 40: 2599-2607. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Ikaros (E-20) X TransCruz antibody is recommended for Gel Supershift and ChIP applications. Try Ikaros (E-2): sc-398265, our highly recommended monoclonal alternative to Ikaros (E-20). Also, for AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647 conjugates, see Ikaros (E-2): sc-398265. Molecular Weight of Ikaros: 50 kDa. Positive Controls: Ikaros (h): 293 Lysate: sc-111057, Jurkat nuclear extract: sc-2132 or U-937 nuclear extract: sc-2156. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



Дата загрузки: 2017-02-23
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..., INC. Ikaros (H-100): sc-13039 BACKGROUND STORAGE Ikaros family members, including Ikaros and... as T cell activation. Helios and Ikaros bind to similar DNA sequences... transcription factors. Members of the Ikaros family contain zinc-finger domains... and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected... and in adult stem cells. Ikaros and Helios also appear to... K – Ikaros 43 K – 34 K – 23 K – Ikaros (H-100): sc-13039. Western blot analysis of Ikaros expression in non-transfected: sc-110760 (A) and human Ikaros transfected... (mouse) mapping to 11 A1. Ikaros (H-100): sc-13039. Immunoperoxidase staining...) program (B). SELECT PRODUCT CITATIONS SOURCE Ikaros (H-100) is a rabbit polyclonal antibody... of Ikaros of human origin. 1. Yamamoto, E., et al. 2005. Ikaros is expressed... hematopoietic zinc-finger transcription factor Ikaros in T cells. FEBS Lett. 579... µg/0.1 ml. 3. Thomas, R.M., et al. 2010. Ikaros silences T-bet expression and interferon.... APPLICATIONS 4. Ma, S., et al. 2010. Ikaros and Aiolos inhibit pre-B-cell...: 4149-4158. Ikaros (H-100) is recommended for detection of Ikaros of mouse... Ikaros targets in colorectal cancer cells. Mol. Cancer Res. 9: 1139-1151. Ikaros (H-100) is also recommended for detection of Ikaros in additional... antibody for Ikaros siRNA (h): sc-35640, Ikaros siRNA (m): sc-35641, Ikaros shRNA Plasmid (h): sc-35640-SH, Ikaros shRNA Plasmid (m): sc-35641-SH, Ikaros shRNA (h) Lentiviral Particles: sc-35640-V and Ikaros shRNA (m) Lentiviral... for use in diagnostic procedures. Ikaros (H-100) X TransCruz antibody is recommended.... Try Ikaros (E-2): sc-398265, our highly recommended monoclonal alternative to Ikaros (H-100...® 647 conjugates, see Ikaros (E-2): sc-398265. Molecular Weight of Ikaros: 50 kDa... nuclear extract: sc-2156, or Ikaros (h): 293 Lysate: sc-111057. Santa...



Дата загрузки: 2017-02-23
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SANTA CRUZ BIOTECHNOLOGY, INC. Ikaros (M-20): sc-9859 BACKGROUND APPLICATIONS Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T-cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. Ikaros (M-20) is recommended for detection of all Ikaros isoforms of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000). REFERENCES 1. Georgopoulos, K., et al. 1992. Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment. Science 258: 808-812. Suitable for use as control antibody for Ikaros siRNA (h): sc-35640, Ikaros siRNA (m): sc-35641, Ikaros shRNA Plasmid (h): sc-35640-SH, Ikaros shRNA Plasmid (m): sc-35641-SH, Ikaros shRNA (h) Lentiviral Particles: sc-35640-V and Ikaros shRNA (m) Lentiviral Particles: sc-35641-V. Ikaros (M-20) X TransCruz antibody is recommended for Gel Supershift and ChIP applications. Molecular Weight of Ikaros: 50 kDa. Positive Controls:Jurkat nuclear extract: sc-2132, Ikaros (h): 293 Lysate: sc-111057, or U-937 nuclear extract: sc-2156 DATA A 2. Molnar, A., et al. 1994. The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins. Mol. Cell. Biol. 14: 8292-8303. 55 K – 3. Sun, L., et al. 1996. Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development. EMBO J. 15: 5358-5369. CHROMOSOMAL LOCATION Genetic locus: IKZF1 (human) mapping to 7p12.2; Ikzf1 (mouse) mapping to 11 A1. SOURCE Ikaros (M-20) is an affinity purified goat polyclonal antibody raised against a peptide mapping at the N-terminus of Ikaros of mouse origin. PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Also available as TransCruz reagent for Gel Supershift and ChIP applications, sc-9859 X, 200 µg/0.1 ml. Blocking peptide available for competition studies, sc-9859 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). STORAGE 34 K – Ikaros (M-20): sc-9859. Western blot analysis of Ikaros expression in non-transfected: sc-110760 (A) and human Ikaros transfected: sc-111057 (B) 293 whole cell lysates. SELECT PRODUCT CITATIONS 1. Payne, K.J., et al. 2001. Cutting edge: predominant expression of a novel Ikaros isoform in normal human hemopoiesis. J. Immunol. 167: 1867-1870. 2. Caballero, R., et al. 2007. Combinatorial effects of splice variants modulate function of Aiolos. J. Cell Sci. 120: 2619-2630. 3. Cho, S.J., et al. 2008. Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: involvement of Ikaros phosphorylation by casein kinase 2. Cell. Mol. Life Sci. 65: 3290-3303. 4. Quirion, M.R., et al. 2009. Cutting edge: Ikaros is a regulator of Th2 cell differentiation. J. Immunol. 182: 741-745. 5. Umetsu, S.E., et al. 2009. Ikaros is a regulator of IL-10 expression in CD4+ T cells. J. Immunol. 183: 5518-5525. Try Ikaros (E-2): sc-398265, our highly recommended monoclonal alternative to Ikaros (M-20). Also, for AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647 conjugates, see Ikaros (E-2): sc-398265. RESEARCH USE For research use only, not for use in diagnostic procedures. 1.800.457.3801 831.457.3800 < Ikaros 43 K – Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. Santa Cruz Biotechnology, Inc. B 132 K – 90 K – fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



Дата загрузки: 2017-02-23
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SANTA CRUZ BIOTECHNOLOGY, INC. Ikaros siRNA (m): sc-35641 BACKGROUND APPLICATIONS Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. Ikaros siRNA (m) is recommended for the inhibition of Ikaros expression in mouse cells. REFERENCES Semi-quantitative RT-PCR may be performed to monitor Ikaros gene expression knockdown using RT-PCR Primer: Ikaros (m)-PR: sc-35641-PR (20 µl, 437 bp). Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. 1. Molnar, A., et al. 1994. The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins. Mol. Cell. Biol. 14: 8292-8303. SUPPORT REAGENTS For optimal siRNA transfection efficiency, Santa Cruz Biotechnology’s siRNA Transfection Reagent: sc-29528 (0.3 ml), siRNA Transfection Medium: sc-36868 (20 ml) and siRNA Dilution Buffer: sc-29527 (1.5 ml) are recommended. Control siRNAs or Fluorescein Conjugated Control siRNAs are available as 10 µM in 66 µl. Each contain a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. Fluorescein Conjugated Control siRNAs include: sc-36869, sc-44239, sc-44240 and sc-44241. Control siRNAs include: sc-37007, sc-44230, sc-44231, sc-44232, sc-44233, sc-44234, sc-44235, sc-44236, sc-44237 and sc-44238. RT-PCR REAGENTS 2. Sun, L., et al. 1996. Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development. EMBO J. 15: 5358-5369. RESEARCH USE 3. Kelley, C.M., et al. 1998. Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 8: 508-515. PROTOCOLS 4. Cortes, M., et al. 1999. Control of lymphocyte development by the Ikaros gene family. Curr. Opin. Immunol. 11: 167-171. See our web site at www.scbt.com or our catalog for detailed protocols and support products. For research use only, not for use in diagnostic procedures. CHROMOSOMAL LOCATION Genetic locus: Ikzf1 (mouse) mapping to 11 A1. PRODUCT Ikaros siRNA (m) is a pool of 3 target-specific 19-25 nt siRNAs designed to knock down gene expression. Each vial contains 3.3 nmol of lyophilized siRNA, sufficient for a 10 µM solution once resuspended using protocol below. Suitable for 50-100 transfections. Also see Ikaros shRNA Plasmid (m): sc-35641-SH and Ikaros shRNA (m) Lentiviral Particles: sc-35641-V as alternate gene silencing products. For independent verification of Ikaros (m) gene silencing results, we also provide the individual siRNA duplex components. Each is available as 3.3 nmol of lyophilized siRNA. These include: sc-35641A, sc-35641B and sc-35641C. STORAGE AND RESUSPENSION Store lyophilized siRNA duplex at -20° C with desiccant. Stable for at least one year from the date of shipment. Once resuspended, store at -20° C, avoid contact with RNAses and repeated freeze thaw cycles. Resuspend lyophilized siRNA duplex in 330 µl of the RNAse-free water provided. Resuspension of the siRNA duplex in 330 µl of RNAse-free water makes a 10 µM solution in a 10 µM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM EDTA buffered solution. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



Дата загрузки: 2017-02-22
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SANTA CRUZ BIOTECHNOLOGY, INC. Ikaros shRNA Plasmid (m): sc-35641-SH BACKGROUND STORAGE AND RESUSPENSION Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T-cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. Store lyophilized shRNA Plasmid DNA at 4° C with desiccant. Stable for at least one year from the date of shipment. Once resuspended, store at 4° C for short term storage or -80° C for long term storage. Avoid repeated freeze thaw cycles. REFERENCES 1. Georgopoulos, K., et al. 1992. Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment. Science 258: 808-812. 2. Molnar, A., et al. 1994. The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins. Mol. Cell. Biol. 14: 8292-8303. 3. Sun, L., et al. 1996. Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development. EMBO J. 15: 5358-5369. 4. Kelley, C.M., et al. 1998. Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 8: 508-515. Resuspend lyophilized shRNA Plasmid DNA in 200 µl of the deionized water provided. Resuspension of the shRNA Plasmid DNA in 200 µl of deionized water makes a 0.1 µg/µl solution in a 10 mM Tris, 1 mM EDTA buffered solution. APPLICATIONS Ikaros shRNA Plasmid (m) is recommended for the inhibition of Ikaros expression in mouse cells. SUPPORT REAGENTS For optimal shRNA Plasmid transfection efficiency, Santa Cruz Biotechnology’s shRNA Plasmid Transfection Reagent: sc-108061 (0.2 ml) and shRNA Plasmid Transfection Medium: sc-108062 (20 ml) are recommended. Control shRNAs are available as 20 µg lyophilized plasmid DNA. Each encodes a scrambled shRNA sequence that will not lead to the specific degradation of any known cellular mRNA. Control shRNA Plasmids include: sc-108060, sc-108065 and sc-108066. RT-PCR REAGENTS Semi-quantitative RT-PCR may be performed to monitor Ikaros gene expression knockdown using RT-PCR Primer: Ikaros (m)-PR: sc-35641-PR (20 µl, 437 bp). Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. CHROMOSOMAL LOCATION Genetic locus: Znfn1a1 (mouse) mapping to 11 A1. PRODUCT Ikaros shRNA Plasmid (m) is a pool of 3 target-specific lentiviral vector plasmids each encoding 19-25 nt (plus hairpin) shRNAs designed to knock down gene expression. Each plasmid contains a puromycin resistance gene for the selection of cells stably expressing shRNA. Each vial contains 20 µg of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also see Ikaros siRNA (m): sc-35641 and Ikaros shRNA (m) Lentiviral Particles: sc-35641-V as alternate gene silencing products. RESEARCH USE The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his laboratory only (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product of its components to a third party, or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



Дата загрузки: 2017-02-23
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SANTA CRUZ BIOTECHNOLOGY, INC. Ikaros shRNA (m) Lentiviral Particles: sc-35641-V BACKGROUND SUPPORT REAGENTS Ikaros family members, including Ikaros and Helios, are nuclear factors that colocalize with DNA replication machinery components in higher-order chromatin structures and respond to signaling events, such as T cell activation. Helios and Ikaros bind to similar DNA sequences, and they function as hemopoietic-specific transcription factors. Members of the Ikaros family contain zinc-finger domains that are involved in DNA-binding and in the formation of homodimers and heterodimers between Ikaros family members. Expression of Ikaros is primarily detected in the thymus and spleen, where it is essential for regulating T-cell specific gene transcription and for the differentiation and commitment of early hemopoietic progenitors to the B and T lymphoid lineages. Similarly, Helios expression is detected primarily in T cells and in the earliest embryonic hemopoietic precursors and in adult stem cells. Ikaros and Helios also appear to regulate cell cycle entry by inducing transcriptional repression under varying conditions and, thereby, mediate T cell activation and lL-2 mediated signaling events. Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an shRNA construct encoding a scrambled sequence that will not lead to the specific degradation of any known cellular mRNA. REFERENCES 1. Georgopoulos, K., et al. 1992. Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment. Science 258: 808-812. 2. Molnar, A., et al. 1994. The Ikaros gene encodes a family of functionally diverse zinc finger DNA-binding proteins. Mol. Cell. Biol. 14: 8292-8303. 3. Sun, L., et al. 1996. Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development. EMBO J. 15: 5358-5369. 4. Kelley, C.M., et al. 1998. Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 8: 508-515. RT-PCR REAGENTS Semi-quantitative RT-PCR may be performed to monitor Ikaros gene expression knockdown using RT-PCR Primer: Ikaros (m)-PR: sc-35641-PR (20 µl, 437 bp). Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. BIOSAFETY Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). Lentiviral particles are replicationincompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. RESEARCH USE The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his laboratory only (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product of its components to a third party, or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. CHROMOSOMAL LOCATION Genetic locus: Znfn1a1 (mouse) mapping to 11 A1. PRODUCT Ikaros shRNA (m) Lentiviral Particles is a pool of concentrated, transductionready viral particles containing 3 target-specific constructs that encode 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see Ikaros siRNA (m): sc-35641 and Ikaros shRNA Plasmid (m): sc-35641-SH as alternate gene silencing products. STORAGE Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to one week. Avoid repeated freeze thaw cycles. APPLICATIONS Ikaros shRNA (m) Lentiviral Particles is recommended for the inhibition of Ikaros expression in mouse cells. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com



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... Island in the Hellenistic period. Ikaros (the city of happiness)………………………………………………………………………………48... Greece, established a port city called Ikaros on Failaka Island. This period... Island in the Hellenistic period. Ikaros (the city of happiness) (Hidden...



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... Anti-Ikaros antibody ab26083 3 Abreviews 5 References 4 Images Overview Product name Anti-Ikaros antibody Description Rabbit polyclonal to Ikaros Tested applications... within residues 1 - 100 of Human Ikaros.Read Abcam's proprietary immunogen policy.... Sequence similarities Belongs to the Ikaros C2H2-type zinc-finger protein... splicing. 2 Anti-Ikaros antibody images All lanes : Anti-Ikaros antibody (ab26083) at... 10 µg per lane. Western blot - Ikaros antibody (ab26083) Secondary IRDye 680... (possible isoform). IHC image of Ikaros staining in human tonsil FFPE...) - Ikaros antibody (ab26083) counterstained with haematoxylin and mounted with DPX. 3 Anti-Ikaros... under reducing conditions. Western blot - Ikaros antibody (ab26083) Predicted band size... a 1/1000 dilution for 1h. Alexa Ikaros antibody (ab26083) Fluor® 594 WGA...