Двигательная установка. Merlin 1. [Редактировать]

ЖРД Merlin-1 компании SpaceX имеет достаточно высокие технические характеристики при относительной простоте конструкции. Он является двигателем открытого (газогенераторного) цикла. Зажигание – пиротехническое. В камере сгорания ЖРД Merlin-1 установлена штифтовая форсунка, аналогичная той, которая впервые была использована в двигателе посадочной ступени лунного модуля КК «Аполлон». Компоненты топлива подаются в камеру сгорания с помощью турбонасосного агрегата (ТНА) с двойной крыльчаткой. ТНА также подаёт керосин под высоким давлением в гидравлическую систему управления вектором тяги. Это исключает необходимость отдельной гидравлической системы управления и гарантирует ее надежное функционирование в течение всего времени работы ЖРД.
Разработанные для РН Falcon-1 и Falcon-1е ЖРД Merlin-1A и Merlin -1C используют для управления по крену поворотный выхлопной патрубок ТНА. ЖРД Merlin-1C для РН Falcon-9 практически идентичны им по конструкции за исключением наличия фиксированной системы выхлопа ТНА. В ЖРД Merlin-1A использовалась простая камера сгорания и сопло с абляционным охлаждением. На ЖРД Merlin-1C установлены регенеративно охлаждаемые сопло и камера сгорания. Первая ступень РН Falcon-9 оснащена девятью двигателями Merlin-1C. Столь значительное их число повышает вероятность выполнения задач полета даже при отказе одного из двигателей.
ЖРД Merlin-1V (Vacuum) разработан для использования на второй ступени РН Falcon-9. Двигатель создан на основе ЖРД Merlin-1C, однако для оптимизации работы в вакууме он имеет бóльшую степень расширения сопла, которое охлаждается теплоизлучением. Диапазон дросселирования тяги ЖРД Merlin-1V составляет 60-100%.


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#Наименование Тип
1Merlin-1CДУ
2Merlin-1C 2ДУ
3Merlin-1DДУ
4Merlin-1VДУ
5Merlin-1АДУ

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Дата загрузки: 2017-12-26
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0.15/5
... MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN GERIN MERLIN...



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0.1/5
.... Seconds before launch, the nine Merlin engines of the first stage... later, the second stage’s single Merlin vacuum engine ignites to begin... diameter). Its nine first-stage Merlin engines generate 1.3 million pounds of... the nine Merlin engines that power Falcon 9’s first stage; one Merlin vacuum.... Falcon 9’s first stage incorporates nine Merlin engines. After ignition, a hold-before... 747s at full power, the Merlin engines launch the rocket to... second stage, powered by a single Merlin vacuum engine, delivers Falcon 9’s payload... and manufacturing efficiencies. Merlin 1D Engine The Merlin engine that powers the... rocket-grade kerosene propellant, a single Merlin engine generates 654 kilonewtons (147...’s atmosphere. Merlin’s thrust-to-weight ratio exceeds 150, making the Merlin the.... 7 The Merlin 1D engine provides a number of improvements over its Merlin 1C... thrust structure of the nine Merlin engines improves upon the former... process. It houses the nine Merlin 1D engines and was designed... in thrust from the Merlin 1C to Merlin 1D engine design. To... failure. Because Falcon has nine Merlin engines clustered together to power...



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0.29/5
... membrane-associated FERMdomain proteins Mer (Merlin) and Ex (Expanded), which are.../ TAZ P Mad F-actin Merlin Kibra AMOT P Expanded Merlin LIN7C FRMD6 NF2 FAT... DPP Ajub PAR3 ZYX NF2 Merlin PAR6 FAT1–4 F-actin αPKC Lats1...-catenin is a tumor neurofibromatosis-2 homologue, Merlin, and the tumor suppressor suppressor...) Kibra is a in conjunction with Merlin and Expanded. Dev Cell 18..., Morrison CD, Gelman IH, Zhang J Merlin and Expanded act through Hippo... P, Anders RA, Pan D (2010) The Merlin/NF2 tumor suppressor functions 99..., Giovannini M, McClatchey AI (2010) Nf2/Merlin controls progenitor homeostasis 117. Matsui...- (2009) Functional inactivation of NF2/merlin in human mesothelioma. tions between...



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0.7/5
Merlin Pro-Программирование и установка Содержание: Введение ............................................................................................................................................... 3 ... особенности............................................................................................................10 Подключение………………………………………………………………………………………….11 -2- Введение: Merlin Pro – это программируемый автономный радиоприемник...-извещателей настраивается на работу с Приемник Merlin Pro имеет 8 выходов (типа сухой... к проводным шлейфам объектового ППК. Устройство Merlin Pro снабжено энергонезависимой буферной памятью... временем их возникновения. Корпус приемника Merlin Pro защищается Tamper-переключателем вскрытия... программирования: Прежде чем приступить к работе Merlin Pro рекомендуется выполнить следующие действия... с назначенными зонами). 4. Расположите извещатели и приемник Merlin Pro как можно ближе к местам... остается неизменным. ◄ ◄ -9- Функциональные особенности: Устройство Merlin Pro выполняет свои функции только... в режиме охраны, в буферную память приемника Merlin Pro будут записываться все тревожные... зафиксировано в буферной памяти событий приемника Merlin Pro. При этом каждую минуту...



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0.05/5
... reactor melusine-2 реактор merlin, юлих EN juelich-merlin reactor РЕАКТОР MH-1A... merlin reactor реактор мерлин, альдермастон 2000-04-12 EN aldermaston reactor merlin реактор мерлин, юлих EN merlin-juelich reactor реактор... 2000-04-12 EN ukaea-merlin reactor РЕАКТОР МИДЛЕНД-1 EN midland...



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0.06/5
... Islander Helicopter Engines Lynx MASC Merlin Puma and Gazelle Sea King...: a Merlin flies over the generic airfield. RIGHT: Caught in a storm. The Merlin... two rotary-wing aircraft – the Merlin and Puma. The filming for... Provost at Cosford, while the Merlin and Puma were captured on...



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0.04/5
...-101 Merlin Joint Supporter EH10 M H3T Scamp SCAM L L1P AW-101 Merlin... B212 L H2T Merlin EH10 M H3T AB-412 B412 L H2T Merlin Joint Supporter... M H3T EH-101 Merlin EH10 M H3T Heliliner EH10 M H3T Merlin EH10 M H3T... Cornell FA62 L L1P Merlin 3 SW3 L L2T Merlin 4 SW4 L/M L2T Merlin 23 SW4 L/M L2T... SW4 L/M L2T SW3 L L2T Merlin 3 SW3 L L2T L2J Merlin 4 SW4 L/M L2T M L2P Metro SW4 L/M L2T PC6T L L1T SA-226TB Merlin...-227TT Merlin 3 SW3 L L2T SA-227AT Expediter SW4 L/M L2T SA-227AT Merlin... Excalibur BE50 L L2P Merlin 2 SW2 L L2T Merlin 3 SW3 L L2T Merlin 4 SW4 L/M L2T Metro SW4 L/M L2T SA-26 Merlin 2 SW2 L L2T SA-226AT Merlin 4 SW4 L/M L2T SA-226T Merlin 3 SW3 L L2T SA-226TB Merlin 3 SW3..., Merlin Joint Supporter H3T M FORNAIRE, F-1 Aircoupe L1P L AGUSTAWESTLAND, EH-101 Merlin Joint Supporter H3T M FORNEY, Aircoupe L1P L AGUSTAWESTLAND, Merlin H3T M FORNEY, F-1 Aircoupe L1P L AGUSTAWESTLAND, AW-101 Merlin H3T M MOONEY... L EHI, EH-101 Merlin H3T M AVIOTECNICA, Exec H1T L EHI, Merlin H3T M CLIFFORD..., E-Z Flyer L1P L NORTHROP, Tigereye L2J M MERLIN, E-Z Flyer L1P L NORTHROP, N-156C Freedom..., Merlin L1P L AERO COMMANDER, 200 Commander 200 L1P L BLUE YONDER, Merlin L1P L AERO COMMANDER, Commander 200 L1P L MACAIR, Merlin L1P L MEYERS, 200 L1P L MERLIN, Merlin L1P L NORTH AMERICAN... L L1P L SWEARINGEN, Merlin 2 L2T L L1P L SWEARINGEN, SA-26 Merlin 2 L2T L CFM, SA-2 Star Streak L1P L FAIRCHILD (1), SA-227TT Merlin... Merlin 3 L2T L SU24 SUKHOI, Su-24 L2J M FAIRCHILD SWEARINGEN, SA-226TB Merlin 3 L2T L SU25 SUKHOI, Scorpion L2J M FAIRCHILD SWEARINGEN, Merlin 3 L2T L SUKHOI, Su-25 L2J M SWEARINGEN, Merlin 3 L2T L SUKHOI, Su-25 Scorpion L2J M SWEARINGEN, SA-226T Merlin 3 L2T L SUKHOI, Su-28 L2J M SWEARINGEN, SA-226TB Merlin 3 L2T... (1), Merlin 23 L2T L/M SUKHOI, Su-33 L2J M FAIRCHILD (1), UC-26 Merlin 4 L2T L/M SUKHOI, Su-27 L2J M FAIRCHILD (1), Merlin 4 L2T L/M SUKHOI... L1P L FAIRCHILD SWEARINGEN, Merlin 4 L2T L/M SUNDERLAND, S-18 L1P L SWEARINGEN, Merlin 4 L2T L/M THORP... Merlin Joint Supporter, AGUSTAWESTLAND EH10 M B36TC Bonanza, BEECH BT36 L H L AW-101 Merlin... L F9F Panther, GRUMMAN F9F L E-Z Flyer, MERLIN EZFL L F-10, CHENGDU J10 M E-Z Harvard..., FAIRCHILD (1) SW4 L/M Merlin, AGUSTAWESTLAND EH10 Mewa, PZL-MIELEC PA34 L Merlin, BLUE YONDER MAME L Mewa M-20, PZL-MIELEC PA34 L Merlin, EHI EH10 Meyer-360, AIRCRAFT TECHNOLOGIES M360 L Merlin, MACAIR MAME L MF-85, RUSCHMEYER R90R L Merlin, AEROCOMP MAME L MFI-9, ANDREASSON JUNR L Merlin, MERLIN MAME L MFI-9 Junior, MALMO JUNR L Merlin 2, SWEARINGEN SW2 L MFI-10 Vipan, MALMO MF10 L Merlin 2 SA-26, SWEARINGEN SW2 L MFI-15 Safari, SAAB MF17 L Merlin 3, FAIRCHILD SWEARINGEN SW3 L MFI-17 Supporter, SAAB MF17 L Merlin 3, FAIRCHILD (1) SW3 L MH-6B, HUGHES H500 L Merlin 3, SWEARINGEN SW3 L MH-6C, HUGHES H500 L Merlin 3 SA-226T, SWEARINGEN SW3 L MH-6E, HUGHES H500 L Merlin 3 SA-226TB, SWEARINGEN SW3 L MH-6H, MCDONNELL DOUGLAS H500 L Merlin 3 SA-226TB, FAIRCHILD SWEARINGEN SW3 L MH-6H, HUGHES H500 L L M M Merlin 3 SA-227TT, FAIRCHILD SWEARINGEN SW3 L MH-6J, MCDONNELL DOUGLAS MD52 Merlin 3 SA-227TT, FAIRCHILD (1) SW3 L MH-02, HONDA-MISSISSIPPI MH02 L Merlin 4, FAIRCHILD SWEARINGEN SW4 L/M MH-46 Eclipse, AEROSETTE MH46 L Merlin 4, FAIRCHILD (1) SW4 L/M MH-47 Chinook, BOEING H47 M Merlin 4, SWEARINGEN SW4 L/M MH-47 Chinook, BOEING VERTOL H47 M Merlin 4 SA-226AT, SWEARINGEN SW4 L/M MH-53E Sea Dragon, SIKORSKY H53S M Merlin 4 SA-227AT, FAIRCHILD SWEARINGEN SW4 L/M MH-53H, SIKORSKY H53 M Merlin 4 SA-227AT, FAIRCHILD (1) SW4 L/M MH-53J, SIKORSKY H53 M Merlin 4 UC-26, FAIRCHILD (1) SW4 L/M MH-60 Black Hawk, SIKORSKY H60 M Merlin 23, FAIRCHILD (1) SW4 L/M MH-60 Knighthawk, SIKORSKY H60 M Merlin Joint Supporter, AGUSTAWESTLAND EH10 M MH-60 Strikehawk, SIKORSKY H60 M Merlin Joint...-226AT Merlin 4, SWEARINGEN SW4 L/M SA-365N Dauphin 2, AEROSPATIALE AS65 L SA-226T Merlin 3, SWEARINGEN SW3 L SA-366 Dolphin, AEROSPATIALE AS65 L SA-226TB Merlin...-227AT Merlin 4, FAIRCHILD SWEARINGEN SW4 L/M Sabre, MITSUBISHI F86 SA-227AT Merlin 4, FAIRCHILD... Merlin 3, FAIRCHILD (1) SW3 L Sabre 65 NA-265, ROCKWELL SBR1 M SA-227TT Merlin... ELICOTTERI MERIDIONALI SPA (ITALY) MERLIN MESSERSCHMITT MERLIN AIRCRAFT INC (UNITED STATES) MESSERSCHMITT...



Дата загрузки: 2017-05-21
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0.14/5
...: Agravaine, Gawain, Guinevere, Isolde, Lancelot, Merlin, Mordred, Pellinore, Tristram. 3 Contents Abstract... of a dream-like afterlife, in Merlin’s cave. Here he is offered...’, and is swiftly admonished by Merlin. In this meta-textual exchange... mercy of superior adult intelligence. Merlin suggests Arthur should feel privileged...’ – which is then qualified by Merlin – ‘you and I know it’ – posits... it among a knowledgeable elite. Yet Merlin’s warning, ‘the people who are... published 1958. 8 ‘stupid about children’, Merlin calls the status of the... acknowledged the influence of his Merlin upon her own Dumbledore. See... out to prove what White’s Merlin observed over four decades previously... children are childish’ – to use Merlin’s words – is the defining practice... alleviate its own ‘degradations’, as Merlin termed them. Lesnik-Oberstein argues... asks Merlin in White’s The Sword in the Stone. ‘Oh dear,’ Merlin..., and to give, unlike White’s Merlin, considered answers to questions about... with King Pellinore. Saved by Merlin’s magic, the King came unto... haste to undertake a dangerous adventure’. Merlin, too, warns Arthur: ‘a real peril... carrion crows, circle ominously overhead. Merlin is dwarfed in the background... a dangerous space, is coupled with Merlin’s warning and the written challenge... face my death,’ he tells Merlin, ‘yet would I not turn back... in seeking it. He disregards Merlin’s warning to turn back, declaring..., such as steadfastness and honour. Merlin tells Arthur that he is... knighthood. 47 87 praise’. Significantly, Merlin advises Arthur, ‘ye owe your..., Pyle’s Arthur is reminded by Merlin ‘thou must bear in mind... situations orchestrated by the magical Merlin. However, as Chapter Three will... 1958. 109 watchful eye of Merlin and his magical powers. Yet...’s childhood under the tutelage of Merlin. Instead, White frequently alludes to... the May Day babies on Merlin’s advice, ‘frightened by horrible prophecies...’s education under the tuition of Merlin. The wizard, as Susan Aronstein... taught to write by Merlin’s owl, Archimedes. Merlin’s magic is a major focal... with an ominous speech from Merlin. ‘I know the sorrows before you... counterbalance Arthur’s joyful education with Merlin. White’s 1958 revised version of... removed the whimsical battle between Merlin and Madam Mim, and, by... completely eluding the child: in Merlin’s remark to Wart that he... face of higher powers: when Merlin declares to the Round Table... asks Merlin why he cannot escape Nimue using his magic arts; Merlin... to rewrite the seduction of Merlin (traditionally identified as the son... Spirits, the Holy Ghost, but Merlin’s pride in himself, which betrayed... other children’s versions, in which Merlin’s fate is portrayed as inevitable...?’, Morgan asks her, referring to Merlin. ‘I have never known what that... behaves recklessly, saved only by Merlin who laments his susceptibility to... teach him anything’, Arthur ignores Merlin’s warnings and ends up drugged... eventually results in censure from Merlin, whose patience is finally exhausted... swearing in turn to give Merlin no further opportunity to gloat... is one of the reasons Merlin succumbs to Vivian’s wiles, deserting..., when Arthur refuses to believe Merlin’s prediction that Guinevere will be..., 77. 28 183 observes that ‘Merlin knew the winding channels of... scene of carnage in which Merlin asks Arthur, ‘Thou has never... respect for, this ‘nonsense’. Steinbeck’s Merlin reprimands Arthur, sulking after losing... childhood: ‘He thought again of Merlin, whom he remembered prophesying about... best knight in the world, Merlin had said’. He had fulfilled... to listen to children,’ Steinbeck’s Merlin tells Arthur in an early... series is not Arthur, not Merlin, not Lancelot, but a group of... de Caldicot seeks counsel from Merlin. ‘You remember we talked about... can happen. Several months previously, Merlin had given Arthur, a young squire... remembers that he once heard Merlin, who exists both inside the... a child of the crossing-places’. ‘Merlin!’ the boy Arthur cries, ‘I’m between... as Merlin’s eyes. ‘I’m at the crossing-places,’ I said. 206 ‘On a quest,’ Merlin... of the legend, particularly Merlin, as in T. A. Barron’s Merlin saga (1996-2000... characters and figures such as Merlin is symptomatic of changing notions.... The successful BBC TV series Merlin (2008-12), as its name... heart of the Arthurian story. Merlin, Arthur and Guinevere are all... ageing Uther Pendragon. It is Merlin’s magic, rather than Arthur’s physical... the obsidian seeing stone from Merlin: When I stared at the stone.... ‘A mirror,’ I said. ‘Not really,’ said Merlin. ‘What is it?’ ‘A gift.’ ‘I mean... for?’ […] ‘That depends on you,’ Merlin said. ‘Only you can tell... his own identity. Rather, through Merlin’s gift of the stone, Arthur... end of Crossley-Holland’s trilogy, Merlin asks the boy Arthur to..., sadly. ‘It is yours,’ said Merlin. ‘Its story will never end... discovered the king in yourself,’ Merlin tells the boy Arthur. ‘You... children’s literature. As Crossley-Holland’s Merlin wisely acknowledges, there is always...”: Malory and the Suite du Merlin.’ In Aspects of Malory, edited...



Дата загрузки: 2017-02-23
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0.15/5
.... He seems oblivious to MERLIN's presence, so MERLIN self-consciously clears his... now in SLOW MOTION: MERLIN TIME. MERLIN glances across the room and... did you just do? MERLIN Uh ... MERLIN EP.1 6. MERLIN looks alarmed, fearing he... of trouble. MERLIN Well ... GAIUS (insistent) Answer me. MERLIN I’ve never studied... truth. MERLIN I was born like this. MERLIN EP.1 7. GAIUS That’s impossible. MERLIN looks... I don’t have my glasses. MERLIN I’m Merlin. GAIUS Hunith’s son? MERLIN Yes. A beat. GAIUS... he’d been standing ... MERLIN EP.1 8. GAIUS Although, Merlin ... MERLIN stops at the door.... GAIUS’ CHAMBERS (MERLIN’S ROOM) - NIGHT 1. UK/FR. 8 A garret room, MERLIN finishes unpacking... give my life that Merlin were not so. MERLIN EP.1 9. HUNITH(cont... the spilt pail is Merlin’s fault) Merlin! MERLIN EP.1 13. MERLIN reacts, turns and... picks up a handful of remedies ... MERLIN MERLIN EP.1 14. GAIUS Here. Hollyhock... MERLIN gets to the door, GAIUS stops him ... GAIUS And Merlin ... MERLIN looks... it. He squints at MERLIN. 20 A MERLIN EP.1 16. MERLIN (holding out bottle... the medicine in one. MERLIN I’m sure it’s fine... MERLIN hurries away. CUT... the courtyard and lands at MERLIN’s feet ... MERLIN EP.1 18. The gang... up. THOMAS looks up at MERLIN. MERLIN (turning to the YOUNG LAD... toward MERLIN ... THOMAS (to MERLIN - not wanting any trouble) It’s alright. MERLIN (refusing... you? MERLIN I’m Merlin. YOUNG LAD So I don’t know you. MERLIN No. MERLIN EP.1 19.... MERLIN can MERLIN That was my mistake. YOUNG LAD I think so. MERLIN Yeah... me to help you? MERLIN’s face hardens MERLIN I wouldn’t if I were you... to do to me? MERLIN EP.1 20. MERLIN You’ve no idea... over MERLIN. step backward ... MERLIN takes a nervous YOUNG LAD Come on, then... MERLIN..., he is in MERLIN TIME... EVERYTHING SLOWS, MERLIN looks at the gang..., calling him, the earth trembles: ‘Merlin’. MERLIN jumps to his feet and... veg. MERLIN Oh, Charlemagne! Everything imaginable is hurled at MERLIN ... As MERLIN takes... Gwen. Right. But most people MERLIN I’m Merlin... MERLIN halfheartedly waggles a hand in the... men and, well ... MERLIN gives her another glance. MERLIN What? GWEN You... would have said? MERLIN absorbs this. GAIUS watches MERLIN eat for a moment... I’ve never seen before. MERLIN EP.1 26. MERLIN Why? GAIUS You promise... has never been posed before, Merlin. MERLIN stares at him, slightly daunted...? MERLIN keeps going. ARTHUR Ah, don’t run away. MERLIN (disparaging) From you? MERLIN turns on him ... MERLIN I’ve told... ... ARTHUR MERLIN EP.1 MERLIN stares at him. 29. There’s no going back ... MERLIN... to MERLIN. MERLIN fumbles it. The gathering CROWD laugh, further angering MERLIN. The... at MERLIN ... MERLIN ducks and comes up wide eyed ... CUT TO: MERLIN EP... rain down, MERLIN suddenly crashes into a fruit stall. MERLIN falls backwards, sending... ... MERLIN Oh ... Charlemagne! We hear MERLIN’s heart beating and we’re in MERLIN TIME... MERLIN’s eyes...’s hammer and aims it at MERLIN. MERLIN steps back, but collides with... magic, what have I got? MERLIN EP.1 33. MERLIN(cont'd) I’m just a ‘nobody’ - and... INT. GAIUS’ CHAMBERS (MERLIN’S ROOM) - DAY 3. UK. MERLIN is lying on his... enter, carrying GAIUS Merlin ... But MERLIN is still angry ... MERLIN Why isn’t there... INT. GAIUS’ CHAMBERS (MERLIN’S ROOM) - NIGHT 3. UK. 39 MERLIN asleep on his... (V.O.) Merlin! MERLIN wakes with a start, unsure if he’s in a dream ... GREAT DRAGON (V.O.) Merlin... he had them slaughtered. MERLIN EP.1 36. MERLIN (appalled) What - all of... and narrower until MERLIN is virtually bent double. Finally, MERLIN turns a corner... destiny. Why? MERLIN What d’you mean? The dragon chortles ... MERLIN What destiny? GREAT DRAGON Your ‘gift’, Merlin, was given... of us choose our destiny, Merlin ... MERLIN EP.1 39. GREAT DRAGON(cont... INT. GAIUS’ CHAMBERS (MERLIN’S ROOM) - DAY 4. UK. 45 MERLIN is asleep, exhausted...? MERLIN (bleary eyed) It just happens. GAIUS By magic? MERLIN Yeah. MERLIN EP... that dress, will you? MERLIN EP.1 41. MERLIN knows he can't afford.... MERLIN EP.1 42. On MERLIN’s face... MORGANA Gwen? GWEN (O.S.) I’m here. A startled MERLIN looks... turns MORGANA away and gives MERLIN a smile. MERLIN quickly scarpers... A relieved MORGANA... a lake nearby... MERLIN nears the sound of a woman singing ... MERLIN peeks through... on me? MERLIN No. (nervous) I was picking herbs. A moment. MERLIN quickly scrambles...’t stand there. Get changed. MERLIN EP.1 MERLIN I’ve just seen Dame Helen... her.. MERLIN is dumbfounded, slack-jawed ... GAIUS (O.S.) (abrupt) Merlin! MERLIN EP.1 45. MERLIN can... bites of food. We see MERLIN’s face... MERLIN EP.1 47. The music... an evil-looking knife. MERLIN EP.1 48. MERLIN stares at the chandelier...: MERLIN EP.1 51 INT. 50. GAIUS’ CHAMBERS (MERLIN’S ROOM) - NIGHT 4. UK. A bewildered MERLIN... Arthur’s life. MERLIN No. GAIUS Perhaps that’s its purpose. MERLIN Please, I hope... and leaves. 51 MERLIN EP.1 51. Intrigued, MERLIN opens the cover. It...



Дата загрузки: 2017-02-23
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0.13/5
... BRAIN A JOURNAL OF NEUROLOGY Neuronal merlin influences ERBB2 receptor expression on... cells demonstrate the influence of merlin on neuregulin 1 type III expression... the first time that axonal merlin indirectly regulates Schwann cell behaviour... have identified that neuronally expressed merlin can influence Schwann cell activity... a cell-extrinsic manner. Keywords: axon; merlin; myelination; neuregulin 1; neurofibromatosis type 2; polyneuropathy... journals.permissions@oup.com Axonal merlin regulates neuregulin 1 type III expression..., the loss of one merlin splice variant, namely merlin isoform 2, results in... leading to neuropathic symptoms in a merlin-mutant mouse model. These findings... obtain conditional, Schwann cell-specific merlin knockout by crossing with the... primary antibodies were used: anti-merlin (1:500, Santa Cruz Biotechnology, clone... 72 C for 1 min. Oligonucleotides Axonal merlin regulates neuregulin 1 type III expression... manufacturer’s instructions to generate C-terminal merlin point mutation carrying mutants Q324L... and subsequently Neuregulin 1 expression in merlin knockout animals 424 | Brain 2014...). We therefore expected that only merlin isoform 2 (merlin-iso2) knockout mice, associated... lysates of both merlin isoform 1 (merlin-iso1)-deficient animals and merlin-iso2-deficient... cellular compartment responsible for the merlin-dependent deregulation of the respective... deletes merlin in Schwann cells (P0-Cre;Nf2flox). The loss of merlin...-specific merlin knockout nerve lysates (Fig. 1B) might be caused by merlin... our observations, we conditionally deleted merlin in the neuronal cell compartment... loading control (n = 3). (B) Loss of merlin-iso1 or merlin-iso2 in vivo leads...-Cre;Nf2 + / + ; n = 3). (E and F) Neuron-specific merlin knockout (Nefh-Cre;Nf2floxfl/fl... of merlin in the neuronal compartment (n = 2). (G) Isoform-specific loss of merlin reduces.... Axonal merlin regulates neuregulin 1 type III expression reduction of merlin gene dosage... of merlin is reflected only by a slight reduction in the overall merlin... merlin mutations in vivo, we studied mice bearing only one functional merlin... one functional merlin allele remains. Loss of one functional merlin allele was... mice (Fig. 1G), indicating that merlin’s involvement in NRG1 expression applies... cross-sections from both merlin-iso2- and merlin-iso1-deficient animals (Fig...;Nf2flox sciatic nerve fibres Figure 2 Merlin knockout animals show altered myelination... of sciatic nerves taken from (B) merlin isoform-specific knockout animals (iso1... expression following merlin overexpression in vitro To further test whether merlin can... of either full length merlin-iso1 or merlin-iso2 in P19 cells... C-terminal fragments of both major merlin isoforms predominantly increased NRG1 type... transfection of different FLAG-tagged merlin fragments. Transfection of empty vector... construct (n = 3). (B) Wild-type merlin isoform 1 (merlin wt) and merlin constructs bearing indicated C-terminal... lysates following overexpression of merlin-iso1 or merlin-iso2 constructs. EGF domain... root ganglion (DRG) cells after merlin overexpression in vitro (n = 3). Blot quantifications... length. tested whether known C-terminal merlin missense mutations derived from patients...-type merlin (Fig. 3B). Taken together, these results verify that merlin can... complete loss of merlin or large truncations of merlin because of nonsense... unchanged following merlin overexpression (Fig. 3C). These findings indicate that merlin does... merlin in primary dorsal root ganglion cells. Overexpression of one major merlin... neurofilaments as an axonal Axonal merlin regulates neuregulin 1 type III expression... specimens of merlin-iso1 knockout mice as representative for merlin loss in... neuronal loss of merlin Upon loss of neuronal merlin a reduction in NRG1... western blotting (Fig. 6C). Axonal merlin regulates neuregulin 1 type III expression... following neuron-specific loss of merlin, whereas NRG1 levels appeared consistently... that merlin downregulation in neurons in both heterozygous and homozygous conditional merlin... nerve lysates of neuron-specific merlin knockout mice showed a reduced activation... expressed merlin modifies Schwann cell activity. Taken together, we link merlin to... the loss of merlin or even reduction of merlin gene dosage in... Axonal merlin regulates neuregulin 1 type III expression reduction of neuronal merlin, which... other neuronal factors affected by merlin independent of the neuregulin network... of ERBB2 expression. Again, whether merlin regulates axonal factors in addition... particularly the loss of axonal merlin increases Schwann cell susceptibility towards... primarily focus on merlin loss in Schwann cells. Neuronal merlin, which is... A, Watilliaux A, Siena M, Chareyre F, et al. Merlin regulates transmembrane receptor accumulation and... NF2 tumor suppressor gene product, merlin, mediates contact inhibition of growth... MJ, Bauer R, Garcia C, et al. Merlin isoform 2 in neurofibromatosis type 2-associated..., Kumar S, Leichsenring G, Morrison H, Baader SL. Merlin inhibits neurite outgrowth in the...