Платформа аппарата. GMP-T. [Редактировать]

Новостная лента[Найти информацию в новостях] [Найти информацию в документах]

#Новостная лента.
12014-12-07. Спутниковый оператор Eutelsat объявил о покупке геостационарного аппарата у SSTL. Спутниковый дизайн аппарата был назван аппаратом Quantum и включает аналоговый бортовой процессор и фазированную антенну от Airbus, а также платформу GPM-T от SSTL.Тэги: Eutelsat Communications S.A.GMP-TSSTL

Технические характеристики

#ХарактеристикаЗначение
1Масса (аппарата на платформе), кг3500
2Масса (полезная нагрузка), кг450
3Мощность, Вт5000

Экономические характеристики

#ХарактеристикаДата измеренияЗначение
1Стоимость (разработки), млн. долл.95

Найдено 1000 документов по запросу «GMP-T». [Перейти к поиску]


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0.1/5
... burglary ................................................................................................... 19 1.4 Greater Manchester Police (GMP) ............................................................................ 22 1.5 The aims and objectives... CSR DAS DCLG DFSC GMP GMP ALU GMP DFSC GMP HQ HMIC LPA NPIA... 1.4 Greater Manchester Police (GMP) Greater Manchester Police (hereinafter GMP) is one of... consists of 12 police divisions (GMP, 2014) 4. Attempting to prevent crime... delivered throughout the force by GMP Design for Security Consultancy (hereinafter... a design led consultancy based within GMP (Monchuk, 2011). Its aim is... conducted a small scale evaluation of GMP’s atypical delivery. At this time... is delivered across Manchester by GMP DFSC. Research Questions: • How is... and commonsense is imperative. 78 GMP has attempted to engage with... forces, since 1990 consultants at GMP have been recruited from a built... how CPTED is delivered across GMP and consider whether a similar approach... thesis. These included: i) ALOs from GMP; ii) ALOs who had a background... purposive sample Stratum 1 ALOs from GMP (i.e. those with a built environment background... a police force that was not GMP Stratum 3 ALOs who were serving..., all the ALOs employed by GMP have been former built environment.... This consisted of: i) four from GMP (stratum 1); ii) one participant from... DFSC liaised with colleagues at GMP Headquarters and attending ‘tour days... introduction of the CIS process, GMP had been delivering CPTED and... still received some involvement from GMP. Third, the age of the... communications which were conducted between GMP and the client when compiling... is delivered across Manchester by GMP DFSC. Semi-structured • Unstructured interviews... protected. All 43 Aside from GMP. 111 digital audio recordings were... 18 months after retiring from GMP. The findings from semi-structured interviews conducted with five GMP consultants and the head of... – a design led consultancy based within GMP. At the time of writing..., Confidence and Equality team of GMP and its head (a civilian) reports... co-located and based at GMP Headquarters. DFSC was previously referred to as GMP Architectural Liaison Unit (hereinafter GMP ALU) and were... estate, but were close to GMP’s Headquarters. As outlined in their... (1994), since 1990 ALOs in GMP have been civilians with a background... Act 1998, Respondent Y stated that GMP engaged with the LPAs in... ad hoc basis. In addition, GMP delivered presentations to built environment... a “catalyst” and instrumental in helping GMP raise the profile of SBD and CPTED amongst LPAs. GMP used the introduction of Section..., had initially received advice from GMP as the developers were seeking... initially sought the advice of GMP, the advised changes were not... in a closer working relationship between GMP ALU and Manchester LPA. Consequently... major planning applications to contact GMP ALU for crime prevention advice... this funding be provided by GMP. Although the Assistant Chief Constable... the planning application. In addition, GMP ALU would offer on-going..., twofold, Respondent Y was adamant that GMP ALU had to be responsible... its residents. Respondent Y questioned why GMP should fund a unit when its... Greater Manchester to pay for GMP to appraise their development in... were not compiled by GMP were identified when GMP was reviewing the.... By reviewing the planning application GMP is able to identify: i) whether... integral to the service which GMP provides. It’s a checking exercise really... quality and speed with which GMP could compile CIS’ and respond... directly responsible to an ACC; GMP being involved from the concept... a completed development and ensuring that GMP provide responses to planning applications... CIS process and liaising with GMP’s legal and finance departments. Respondent... Nationally as well as across GMP. 199 Nevertheless, Respondent Z was hopeful... be tasked with liaising with GMP colleagues and identifying sources of... 6 4 4 3 3 3 1 1 During the period of analysis, GMP responded to only one call... the Architectural Liaison Officer from GMP. The cycle path link now... burglary offences were recorded by GMP during the period of analysis... CIS e.g. Design for Security, GMP emergency planning, GMP counter terrorism unit; and... quotation: I think this was when GMP were thinking well how can... it doesn’t come in from GMP, because that’s monopolising and until... and disorder issues across Manchester, GMP is the most resourceful owing... process. DFSC is governed by GMP. Whilst this is advantageous, as... was a freeze on recruitment across GMP. This raises issues regarding the... the delivery of CPTED across GMP. 300 This research has confirmed... approach similar to that at GMP, especially when policing budgets are... [online] Available at: http://www.gmp.police.uk/live/nhoodv3.nsf... site visit discussion with another GMP ALO i) other (please state) 8. Where... to the Greater Manchester Police (GMP) Design for Security (DFS) team... of the Greater Manchester Police (GMP) Design for Security (DFS) team...



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... Meetings 66 67 67 Appendix 1 GMP Model Rules 69 Appendix 2 Scheme... provisions of Appendix 1 hereto (the GMP Model Rules) shall apply if...-out Provisions 183 OVERRIDING APPENDIX - GMP MODEL RULES CONTENTS Page Clause... to GMP Guaranteed Minimum Member's GMP Widow's GMP Payment of Widow's GMP Widowers GMP Payment of Widower's GMP Civil Partner's GMP Payment of Civil Partner's GMP Offsetting pension against GMP 6. Revaluation of GMP Revaluation... in 6.2 Transfers out 6.3 7. Increase of GMP Increase in event of postponement... Scheme Conditions for transfer of GMP's 10.1 10.2 Effect of such... 11. Commutation of GMP 12. Forfeiture of GMP 13. Contributions Equivalent Premiums..." means the method of revaluing a GMP before State Pension Age described in Rule 6.1(B) below. "GMP" means the guaranteed minimum pension..." means the method of revaluing a GMP before State Pensionable Age described... so long as anyone has a GMP or a prospective right to receive a GMP under the Scheme. This Appendix ... Rule are satisfied. 5. ENTITLEMENT TO GMP 5.1 Guaranteed Minimum This Rule 5 applies... of the 1993 Act. 5.2 Member's GMP The Member shall be entitled... to the postponement 72 5.3 Widow's GMP Where a Member is a man and... guaranteed minimum. 5.4 Payment of Widow's GMP The pension shall be payable... of those State benefits. 5.5 Widowers GMP Where the Member is a woman... 1996/97. 5.6 Payment of Widower's GMP The pension shall be payable... is eligible for payment of a GMP under Regulation 57 of the... to receive payment of that GMP under Regulation 58 of those Regulations. 5.6A Civil Partner's GMP Where the Member dies leaving.... 5.6B Payment of Civil Partner's GMP The pension shall be payable... is eligible for payment of a GMP under Regulation 57 of the... to receive payment of that GMP under Regulation 58 of those Regulations. 5.7 Offsetting pension against GMP Any pension payable to the... (see Rule 8 below). REVALUATION OF GMP Revaluation before State Pension Age... State Pension Age, the Member's GMP at State Pension Age or... increasing the accrued rights to GMP at cessation of Contracted-out... rights of the Member to a GMP, (or includes protected rights in... the receiving scheme will provide a GMP) the earnings factors used in calculating that GMP will normally be revalued using... that scheme that the Member's GMP shall, instead of being revalued... terms as Rule 6.2. 7. INCREASE OF GMP 7.1 Increase in event of postponement... the commencement of any Member's GMP is postponed for any period after State Pension Age, that GMP shall be increased to the... Age or Members death. Any GMP to which a Member, Widow, Widower...'s, Widow's, Widower's or Civil Partner's GMP under Rule 6 or Rule 7. 9. TRANSFERS... to have any entitlement to a GMP under the Scheme. If the... the fact that the Member's GMP rights have been transferred. 11. COMMUTATION OF GMP A Members, Widow's, Widower's or Civil Partners GMP may be... expenses. 13. SUSPENSION OF GMP Payment of a GMP may be suspended during... when (1) the person receiving the GMP is unable to act (by...) but the amount of the GMP must either be paid or... (2) the Member is receiving the GMP but is then re-employed... which the Scheme relates. The GMP must then be increased under.... 14. FORFEITURE OF GMP Any instalment of a GMP may be forfeited if..., nor in respect of Widower's GMP where the Member is a woman... the receiving scheme will provide a GMP)" from Rule 6.2; and (iii) 7. Rule...



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0.42/5
...: общее Руководство ВОЗ по требованиям GMP – надлежащей практике организации производства Часть...: общее Руководство ВОЗ по требованиям GMP – надлежащей практике организации производства Часть... надлежащей практике организации производства (требованиям GMP) Часть 1. Стандартный порядок действий и производственный... руководства …………………………………..……………… 2. Надлежащая практика организации производства (GMP) …………………………… 3. Управление качеством …………………………………………………………….………. 4. Документация ……………………………………………………………………………….. 4.1 Стандартный порядок... 3: Приложение 4: Приложение 5: Приложение 6: 4 Сокращения EP: GMP: ПР: QA: QC: QO: СПД... (ВОЗ) к надлежащей практике организации производства (GMP), причем при работе над данным... своды Правил/Методических рекомендаций по GMP и публикации на данную тему. Библиографический... документа, соответствуют требованиям ВОЗ в отношении GMP. В Руководстве кратко изложен диапазон «письменных... валидации. 6 2. Надлежащая практика организации производства (GMP) Согласно определению, предложенному ВОЗ, под надлежащей практикой (организации) производства (GMP) подразумевается «тот объем мероприятий по... с такой продукцией» (поз. 27). Правила GMP касаются всех аспектов процесса производства... препаратов и рассмотрения жалоб. Руководящий принцип GMP состоит в том, что качество закладывается... промышленности рассмотрены в Руководстве ВОЗ по GMP для фармацевтических препаратов (поз. 27... «обеспечением качества». Концепции обеспечения качества, GMP и контроля качества представляют собой взаимосвязанные... призваны обеспечить следующее: соблюдение правил GMP и других сводов нормативных требований (Надлежащей... всех Правилах или Руководствах по GMP неизменно подчеркивается исключительная важность статуса... качества, в том числе соблюдение правил GMP и контроля качества, осуществляются должным образом... работу фармацевтической компании согласно требованиям GMP. Основная цель системы разработанной или... других национальных и международных Руководствах и Правилах GMP (поз. 3, 5, 7, 11, 18, 19), подчеркивается... всему тексту Руководства ВОЗ по GMP наряду с разделами, посвященными требованиям к оформлению.... Во всех документах Руководств и Правил GMP по фармацевтическим препаратам, стерильным изделиям.... В результате обзора рекомендованных ВОЗ требований GMP на предмет ссылок на «письменные... на предприятии, где соблюдаются правила GMP. В документации такого рода представлено точное... зрения организации производства по правилам GMP). Стандартные учетные формы заполняются техническим... (соответствующие Национальные или Международные правила GMP) Наименование предприятия: Компания по производству... качества с точки зрения соответствия требованиям GMP, нормативным требованиям и требованиям, предъявляемым к качеству... обеспечением качества 6.4 Соответствующие правила и нормы GMP, касающиеся внутренних аудиторских проверок и внешних... обеспечить соответствие рекомендуемым ВОЗ правилам GMP. 2. Предназначение Надзор за производственной деятельностью... СПД. - Методические рекомендации ВОЗ по GMP. (iii) СПД: БИОЛОГИЧЕСКИЕ ИСХОДНЫЕ МАТЕРИАЛЫ... биологических препаратов. Во всех правилах GMP приведены конкретные параметры важнейших асептических... производственных помещений. Рекомендации ВОЗ по GMP для фармацевтических препаратов, TRS 823... ВОЗ и другими странами документах по GMP разработка и утверждение производственного регламента считаются... производственного процесса. В различных документах по GMP варианты толкования термина «производственный регламент... регламент» (master formulae); в США в правилах GMP это понятие трактуется как «основной... processing instructions), а в австралийском варианте правил GMP это понятие дается как «производственный... часть информации, которая изложена в документах GMP, в сущности, остается неизменной. Производственный регламент... национальных и международных Правил и Руководств по GMP (поз. 3, 5, 7, 11, 18, 19), ясно... им с тем, чтобы соответствовать нормам GMP. Слово «формализованный» встречается неоднократно и касается..., чтобы соответствовать предлагаемым ВОЗ Правилам GMP. При необходимости разработать целую серию... и обучение персонала (по техническим вопросам и GMP) Составление и утверждение СПД Сообщения о побочных... обучения персонала MPHBL по вопросам GMP Программа профессиональной подготовки персонала MPHBL... Directorate Guidelines. Good Manufacturing Practices (GMP) Guidelines, Consultation Draft Fourth Edition... качества (quality control) – раздел правил GMP, касающихся выборочного контроля, технических условий... бактериологических лабораторий по действующим правилам GMP ………………………………………...…. 3) Микробиологический мониторинг внешней среды в процессе... бактериологических лабораторий по действующим правилам GMP _____________________________________________________________________________ МАССАЧУСЕТСКИЕ САНИТАРНО-БАКТЕРИОЛОГИЧЕСКИЕ ЛАБОРАТОРИИ... бактериологических лабораторий по действующим правилам GMP I. ЦЕЛЬ: Цель данного СПД состоит... регулярно проводимых курсов подготовки по GMP для персонала всех структурных подразделений... деятельности Участие в дискуссии по вопросам GMP Выполнение роли ведущего по вопросам...



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... image to a Gray-Matter Probability (GMP) map which is coregistered into...: 1. In anatomically homogenous areas (i.e. similar GMP across subjects), tissue type will... the functional data by the GMP only (the reduced model) is... linux-based desktop computer. The GMP for each subject is read...) GLM analysis. Gray-Matter Probability (GMP) maps T1-weighted MRI images... segmentation. The Gray-Matter Probability (GMP) maps were masked (i.e. voxels outside... PET:4.80; rhesus GMP:6.27; human fMRI and GMP:9–10. Prior to groupwise analysis, the GMP images were zero-meaned, i.e. the mean GMP map calculated... from each subject's GMP map. The resulting voxelwise GMP covariates (x'j from Eq... for between-group difference in GMP. (b) Results for a standard GLM betweengroup... remaining after modeling the voxelwise GMP covariate. The second model performs... different effects of incorporating voxelwise GMP values as covariates: 1. A functional activation... since there is only a small GMP effect. Table 1 Summary of rhesus... clusters Coordinates (mm from AC) GMP t-test Standard GLM t-test GLM with voxelwise GMP covariates F-change Putamen Area 13... maximum, the corresponding VBM results (“GMP t-test”) were extracted, as were... in Eq. (2) (“GLM with voxelwise GMP covariates”) and the associated F-change... magnitude and size when voxelwise GMP covariates were included in the..., but became quite significant when GMP covariates were included in the... below the significance level when GMP covariates were included, indicating that.... Left column: t-statistic map for GMP differences between groups showing a two... with voxelwise Gray Matter Probability (GMP) covariates included in the model... both size and magnitude with GMP voxelwise covariates. Bottom row: the... below the significance level when GMP voxelwise covariates are included in... clusters Coordinates (mm from AC) GMP t-test Standard GLM t-test GLM with voxelwise GMP covariates F-change Figs. 2a–c Figs... the GM segment, so the GMP covariates had little effect on... hoped-for correction based on GMP does not occur, but in... match the smoothness of the GMP data to that of the... of autocorrelation across the image. GMP data which are undersmoothed, due... subsequent processing steps. If the GMP data are much smoother than... in reduced sensitivity of the GMP correction. By matching the smoothness.... Intriguingly, the use of voxelwise GMP covariates is a specific instance of... covariates, in this case the GMP maps, requiring critical evaluation of...



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0.83/5
..., 3100, 3700 мм NEW! GLOBAL GMP INDUSTRIAL Серия прессов для средних... мм 32 точки откачки GLOBAL GMP PROFESSIONAL Серия прессов для небольших..., обрабат. мм детали, мм GLOBAL GMP-STANDARD Экономичная серия прессов. Каучуковые... (P) Stationary (S) наименование габариты зоны, см GMP-1313-00-S 130 х 130 RU 0 150 10770 7820 GMP-1313-25-S 130 х 130 PU 250 325 11070 8110 GMP-1313-50-S 130 х 130 PU 500 600 11380 8400 GMP-2513-00-S 250 х 130 RU 0 150 13470 10110 GMP-2513-25-S 250 х 130 PU 250 325 13820 10550 GMP-2513-50-S 250 х 130 PU 500 600 14170 10900 GMP-3113-00-S 310 х 130 RU 0 150 14650 11380 GMP-3113-25-S 310 х 130 PU 250 325 15040 11770 GMP-3113-50-S 310 х 130 PU 500 600 15420 12170 GMP-3713-00-S 370 х 130 RU 0 150 15840 12570 GMP-3713-25-S 370 х 130 PU 250 325 16260 13000 GMP-3713-50-S 370 х 130 PU... Мембраны с рамами для вакуумных прессов GMP 1313, 2513, 3113 & 3713 цена... детали, мм GMP-1313-00-TF GMP-1313-25-TF GMP-1313-50... 850 1150 GMP-2513-00-TF GMP-2513-25-TF GMP-2513-50... 1470 1850 GMP-3113-00-TF GMP-3113-25-TF GMP-3113-50... 1350 1750 2150 GMP-3713-0-TF GMP-3713-25-TF GMP-3713-50...



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0.28/5
... сопутствующих мероприятий для их поддержки. GMP требует от производителя осуществлять мероприятия... обеспечения достоверности полученных результатов. Правила GMP рекомендуют проводить валидацию, в первую очередь... международного стандарта ISO 9001 и правил GMP ЕС. Обоснована целесообразность регламентации процесса внутреннего аудита в соответствии с нормативами GMP и положениями стандартов ISO 9001 и ISO... accordance with the standards of GMP and the provisions of the... международного стандарта ISO 9001 и требования GMP ЕС). В качестве информационной базы исследований...-ваны требования нормативных актов по GMP, стандарты ISO серии 9000, а также... соответствия СМК. В соответствии с требованиями Руководства GMP EC [1] на производственных фармацевтических предприятиях... самостоятельные проверки соответствия правилам стандартов (GMP/ISO), а также принятие необходимых предупреждающих... и выполнением правил надлежащей производственной практики (GMP), а также внесением предложений относительно необходимых... было упомянуто выше, в Ру-ководстве GMP EC [1] также указано, что проведение... на ФП, предусмотренный ISO 9001 и GMP, является рацио-нальным шагом к налаживанию... standard ISO 9001 and EU GMP requirements are shown. The feasibility... accordance with the standards of GMP and the provisions of the...



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.../journal/molecules Article Endo-S-c-di-GMP Analogues-Polymorphism and Binding Studies... / Published: 9 November 2012 Abstract: C-di-GMP, a cyclic guanine dinucleotide, has been... simultaneously modified (called endo-S-c-di-GMP analogues). We used computational methods... with Vc2 RNA (a class I c-di-GMP riboswitch) revealed that conservative modifications... class I riboswitch. Keywords: c-di-GMP; endo-S-c-di-GMP; polymorphism; G-quadruplex; analogues; DOSY... 1. Introduction Cyclic diguanylic monophosphate (c-di-GMP) is a second messenger in bacteria... 1. Family of dual-modified c-di-GMP analogues synthesized. Importantly, endo-S-c-di... to selectively target c-di-GMP receptors. C-di-GMP has also been shown... that the 2'-modification of c-di-GMP differentially affected binding of these... 2.1. Synthesis of c-di-GMP Analogues Endo-S-c-di-GMP analogues 3–5 were easily synthesized.... Scheme 1. Synthesis of endo-S-c-di-GMP analogues (2–5). Reagents and conditions: (a) done... h. 2.2. Polymorphism of c-di-GMP Analogues Polymorphism of c-di-GMP, which depends on... states of c-di-GMP. For example, c-di-GMP binds to most I-sites... selectively target c-di-GMP receptors with c-di-GMP-like molecules that mainly... of both c-di-GMP (1), endo-S-c-di-GMP (2) and 2'-F-endo-S-c-di-GMP (5) seem to... conformers, whereas for 2'-H-endo-S-c-di-GMP (3) the energies of both conformers... all of the possible c-di-GMP conformers. Additionally this calculation might... endo-S-c-di-GMP (2) whereas 2'-H-endo-S-c-di-GMP (3) and 2'-OMe-c-di-GMP (4) would behave differently from endo-S-c-di-GMP...-GMP (1) and analogues (2–5). c-di-GMP (1) endo-S-c-di-GMP (2) 2'-H-endo-S-c-di-GMP (3) 2'-OMe-endo-S-c-di-GMP (4) 2'-F-endo-S-c-di-GMP... equation ΔE = −RTlnK (T = 298 K). When c-di-GMP forms G-quadruplexes, each monomer unit... diffusion constants of c-di-GMP (1) and endo-S-c-di-GMP (2) [27]. Because an... the propensities of 2′-H-endo-S-c-di-GMP (3) and 2′-OMe-endo-S-c-diGMP (4) to... lower than for c-di-GMP (1) and endo-S-c-di-GMP (2) (see Figures 2b... KCl, 30 °C. (c) 3.0 mM 2'-H-endo-S-c-di-GMP, no metal cation, 30 °C. (d) 3.0 mM..., 30 °C. (c) 3.0 mM 2'-OMe-endo-S-c-di-GMP, no metal cation, 30 °C. (d) 3.0 mM... DOSY experiments). However, 2'-F-endo-S-c-di-GMP (5) formed higher aggregates (octamers) in... KCl, 30 °C. (c) 3.0 mM 2'-F-endo-S-c-di-GMP, no metal cation, 30 °C. (d) 3.0 mM... K+ to a solution containing 2'-F-endo-S-c-di-GMP (5), more peaks appeared near 8.00....92 Å) of octameric 2'-F-endo-S-c-di-GMP and hence provides additional evidence... of potassium to 2'-F-endo-S-c-di-GMP promotes aggregation into octameric forms... previously shown that endo-S-c-di-GMP (2) does not readily form G-quadruplexes and commented that c-di-GMP G-quadruplex formation is sensitive to... to disrupt c-di-GMP signaling in bacteria. C-di-GMP has been shown... form (see Figure 5). Figure 5. c-di-GMP fluorescent riboswitch. Sequence of RNA... module) Transduction  module DFHBI = C‐di‐GMP In a detailed study by Strobel... competition with radio-labeled c-di-GMP [28], equilibrium microdialysis [33] and...-GMP or analogue. The fluorescent c-di-GMP sensor would bind to c-di-GMP...: c-di-GMP (1) > endo-S-c-di-GMP (2) > 2'-F-endo-Sc-di-GMP (5) > 2'-H- and 2'-OMe-endo-S-c-di-GMP... 6. Differential binding of endo-S-c-di-GMP analogues to fluorescent Vc2 RNA... [43]. Conditions: [RNA, SP_2] = 1 µM, [c-di-GMP and/or analogues] = 10 µM. Buffer... 0 c-di-GMP (1) no target endo-S-cdi-GMP (2) 2’-H-endo-S-cdi-GMP (3) 2’-OMe-endo-S-cdi-GMP (4) 2’-F-endo-S-cdi-GMP (5) 3. Experimental... a stock solution of c-di-GMP and c-di-GMP analogues was determined Molecules... 253 nm for c-di-GMP and c-di-GMP analogues, using 28,600... for Spectrometric Measurements C-di-GMP or c-di-GMP analogues, water, and metal... Preparation of Fluorescence Measurement C-di-GMP or analogues and DFHBI concentrations... with the transcription of c-di-GMP fluorescent riboswitch. References and Notes...–281. 2. Römling, U.; Gomelsky, M.; Galperin, M.Y. C-di-GMP: The dawning of a novel bacterial..., 929–948. 6. Römling, U. Cyclic di-GMP, an established secondary messenger still... with second messenger cyclic di-GMP suggest catalytic mechanism of phosphodiester... PA4608 complex with cyclic di-GMP identifies charge clustering as molecular... from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor. Mol. Microbiol..., K.D.; Strobel, S.A. Interactions of the c-di-GMP riboswitch with its second messenger... 2'-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins..., M.T.; Chou, S.H. Structural polymorphism of c-di-GMP bound to an EAL domain.... 37. Zhang, Z.; Gaffney, B.L.; Jones, R.A. C-di-GMP displays a monovalent metal ion-dependent... the bacterial second messenger c-di-GMP: Reaction rates and equilibrium constants..., H.O. Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy. Chem...



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... obtained by multiplying the weekly GMP figures by 52. Calculation of... GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI) CP SUR PRE GMP POST GMP... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI) CP SUR PRE GMP POST GMP... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI) + Adj B CP SUR PRE GMP POST GMP NI MOD Adj B Fp Fsur... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... figure. Similarly, the GMP amounts will be the GMP accrued during active... should exclude any transferred-in GMP. Then TVActSer can be calculated... pension sharing 2.2.16 Value of GMP benefits Receiving pension arrangements may... GMP is calculated using the following formula: [PRE GMP + (G x POST GMP)] x FGMPval PRE GMP POST GMP FGMPval G annual GMP accrued before 6 April 1988 annual GMP... conversion factor applied to GMP valuation factor, for GMP accrued in respect... value of the GMP benefits for females, the GMP amounts should be...) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) + Adj B CP SUR PRE GMP POST GMP Adj...-retirement marriage, see section 3.3.6 annual GMP accrued before 6 April 1988, including revaluation; see section 3.3.12 annual GMP accrued from 6 April 1988, including... conversion factor applied to GMP deduction, for GMP amount in respect of... on the Guaranteed Minimum Pension (GMP) that are the responsibility of... values. Where the GMP is in payment, the GMP amounts used in... obtained by multiplying the weekly GMP figures by 52. 3.3.14 Adjustment... applying to the GMP will be: PREGMPDEB = PRE GMP x appropriate percentage  100 POSTGMPDEB = POST GMP x appropriate percentage  100 CP, SUR, PRE GMP and POST GMP are defined in section... x appropriate percentage  100 PREGMPDEB = PRE GMP at exit x appropriate percentage  100 POSTGMPDEB = POST GMP at exit x appropriate percentage  100...  appropriate percentage  100 PREGMPDEB = PRE GMP used in cash equivalent calculation  appropriate percentage  100 POSTGMPDEB = POST GMP used in cash equivalent calculation... Reckonable service Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A1 note 1) Pre GMP & Post GMP = 0; so FGMP is not... Police Authority Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A2 note 1) Pre GMP & Post GMP = 0; so FGMP is not... Police Authority Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... Reckonable service Pre 6/4/88 GMP Post 6/4/88 GMP NI Mod Member’s aggregate... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... not required because PRE GMP and POST GMP are both zero FNI... Reckonable Service1 Pre 6/4/88 GMP Post 6/4/88 GMP Pension increases factor 14... Reckonable service1 Pre 6/4/88 GMP Post 6/4/88 GMP NI Mod Member’s aggregate... x Fp)+ (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI...  appropriate percentage  100 PREGMPDEB = PRE GMP used in cash equivalent calculation... and pension sharing POSTGMPDEB = POST GMP used in cash equivalent calculation... Survivor’s pension Pre 6/4/88 GMP Post 6/4/88 GMP G. Pension increase factor since... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A1 note 1) Pre GMP & Post GMP = 0; so FGMP will not... Survivor’s pension Pre 6/4/88 GMP Post 6/4/88 GMP 11 June 1973 12... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) + Adj B We have... (from Table H2 note 1) Pre GMP & Post GMP = 0; so FGMP will not... 1 When calculating the deduction for GMP, the factor given should be.... 2 When calculating the value of GMP rights, the factor given should... 1 When calculating the deduction for GMP, the factor given should be.... 2 When calculating the value of GMP rights, the factor given should... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be... 1 When calculating the deduction for GMP, the factor given should be...



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... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... or A2 Ar ch PRE GMP POST GMP NI MOD Fp Fsur... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI...) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI) PRE GMP POST GMP NI... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... B CP SUR Ar ch PRE GMP POST GMP NI MOD Adj B Fp... spouse or civil partner annual GMP accrued before 6 April 1988 annual... conversion factor applied to GMP deduction, for GMP amount in respect of... figure. Similarly, the GMP amounts will be the GMP accrued during active... should exclude any transferred-in GMP. Then TVActSer can be calculated... pension sharing 2.2.16 Value of GMP benefits Receiving pension arrangements may... GMP is calculated using the following formula: [PRE GMP + (G x POST GMP)] x FGMPval PRE GMP POST GMP FGMPval G annual GMP accrued before 6 April 1988 annual GMP... conversion factor applied to GMP valuation factor, for GMP accrued in respect... value of the GMP benefits for females, the GMP amounts should be... pension increase uprating factors. The GMP debits (PREGMPDEB and POSTGMPDEB) should... x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) + Adj B Ar PRE GMP current member...-retirement marriage, see section 3.3.6 annual GMP accrued before 6 April 1988, including revaluation; see section 3.3.12 annual GMP accrued from 6 April 1988, including... conversion factor applied to GMP deduction, for GMP amount in respect of... on the Guaranteed Minimum Pension (GMP) that are the responsibility of... values. Where the GMP is in payment, the GMP amounts used in... obtained by multiplying the weekly GMP figures by 52. 3.3.14 Adjustment... applying to the GMP will be: PREGMPDEB = PRE GMP x appropriate percentage  100 POSTGMPDEB = POST GMP x appropriate percentage  100... ed CP, SUR, PRE GMP and POST GMP are defined in section... x appropriate percentage  100 PREGMPDEB = PRE GMP at exit x appropriate percentage  100 POSTGMPDEB = POST GMP at exit x appropriate percentage  100...  appropriate percentage  100 PREGMPDEB = PRE GMP used in cash equivalent calculation... percentage  100 ch POSTGMPDEB = POST GMP used in cash equivalent calculation... 1.3. 3.7.31 Pension debits – GMP debits At the member’s GMP pension age (or later retirement date) the GMP debit, calculated in the appropriate... deducted from the member’s full GMP. 3.7.32 Pension debits – other benefits... Reckonable service Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A1 note 1) Pre GMP & Post GMP = 0; so FGMP is not... Police Authority Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A2 note 1) Pre GMP & Post GMP = 0; so FGMP is not... Police Authority Pre 6/4/88 GMP Post 6/4/88 GMP Have the figures in... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... Reckonable service Pre 6/4/88 GMP Post 6/4/88 GMP NI Mod Member’s aggregate... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... not required because PRE GMP and POST GMP are both zero FNI... Reckonable Service1 Pre 6/4/88 GMP Post 6/4/88 GMP Pension increases factor Ar... Reckonable service1 Pre 6/4/88 GMP Post 6/4/88 GMP NI Mod Member’s aggregate... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI...  appropriate percentage  100 PREGMPDEB = PRE GMP used in cash equivalent calculation  appropriate percentage  100 POSTGMPDEB = POST GMP used in cash equivalent calculation... Survivor’s pension Pre 6/4/88 GMP Post 6/4/88 GMP G. Pension increase factor since... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table A1 note 1) Pre GMP & Post GMP = 0; so FGMP will not... Survivor’s pension Pre 6/4/88 GMP Post 6/4/88 GMP 11 June 1973 12... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) + Adj B We have... Table H2 note 1) Ar Pre GMP & Post GMP = 0; so FGMP will not... Survivor’s pension Pre 6/4/88 GMP Post 6/4/88 GMP G. Pension increase factor since... x Fp) + (SUR x Fsur) – ([PRE GMP + (Gpost88 x POST GMP)] x FGMP) – (NI MOD x FNI... (from Table D2 note 1) Pre GMP & Post GMP = 0; so FGMP will not... 1 1 When calculating the deduction for GMP, the factor given should be... 1 1 When calculating the deduction for GMP, the factor given should be... 1 1 When calculating the deduction for GMP, the factor given should be... 1 1 When calculating the deduction for GMP, the factor given should be... 1 1 When calculating the deduction for GMP, the factor given should be...



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... the Bacterial Second Messenger c-di-GMP: Reaction Rates and Equilibrium Constants... * ABSTRACT: Cyclic diguanosine-monophosphate (c-di-GMP) is a bacterial signaling molecule that... persistence of infection. Previously, c-di-GMP has been reported to display... observed for a circular trinucleotide of GMP.22 The higher oligomeric species... three-dimensional models of c-di-GMP. (A) Chemical structure and atom numbering of monomeric c-di-GMP in anti conformation. (B) Crystal structure....5 (C) Modeled structure of tetrameric c-di-GMP forming two G-quartets in anti... yellow sticks for clarity. c-di-GMP concentrations, the presence of G-quartets... determine the state of c-di-GMP at physiological, i.e., micromolar, concentrations in... and equilibrium constants for c-di-GMP monomer, dimer, tetramer, and octamer... A recent study revealed that c-di-GMP polymorphism critically depends on the... Modeling of c-di-GMP Oligomers. Models of c-di-GMP tetramers and octamers..., respectively). Sample Preparation. Purified c-di-GMP was produced as described previously... (volume >400 μL) for low c-di-GMP concentrations (<2 mM) or Shigemi tubes... 15 h, depending on the c-di-GMP concentration (∼10 mM to ∼0.5 μM, respectively... water (pH 7). Determination of c-di-GMP Concentrations. Due to the hypochromic... upon oligomerization (see below), c-di-GMP concentrations were determined not by... M−1 cm−1.32 The investigated c-di-GMP concentration ranges were 0.5 μM−6 mM (buffers.... Determination of c-di-GMP Dimer Dissociation Constants. C-di-GMP monomer and dimer... below), the total c-di-GMP concentration [c-di-GMP]t was adjusted for each... UV absorption spectrum A(λ) of a c-di-GMP sample after dilution was modeled... environment with respect to c-di-GMP concentration, salt, pH, and temperature... characterization of c-di-GMP-related enzymes. C-di-GMP under Physiological Salt and... At a concentration of 1 mM, c-di-GMP exhibits several resonances for the... remain constant with varying c-di-GMP concentration, whereas their relative intensities... H1′ resonances of 1.0 mM c-di-GMP dissolved in buffer A. Resonance assignments... various c-di-GMP concentrations in buffer A. Indicated c-di-GMP concentrations were obtained... TRIS Buffer. To study c-di-GMP oligomerization behavior also for conditions... to induce oligomerization of c-di-GMP at low millimolar concentrations, although... function of the total c-di-GMP concentration for buffers A and B, respectively... about 20− 100 μM total c-di-GMP concentration (depending on buffer conditions... 3. Concentration dependence of the c-di-GMP monomer/ dimer equilibrium as evidenced... function of total c-di-GMP concentration [c-di-GMP]t (left) and combined monomer... S7, Table S8). Higher c-di-GMP Oligomers. Consistent with the previously...-dependent polymorphism21 at millimolar c-di-GMP concentrations, several sets of resonances... and Equilibrium Constants of c-di-GMP Oligomerization 2M ⇄ D buffer KMD [10... fixed during fit. Figure 4. C-di-GMP oligomer reassociation after heat dissociation... American Chemical Society Article di-GMP enzymatic or biological assays, and... on thiophosphate analogues of c-di-GMP under different buffer conditions.36... experiment. For this, a 280 μM c-di-GMP sample dissolved in buffer A was... for in vitro c- Figure 5. C-di-GMP oligomer dissociation upon dilution. A times... determined concentrations of various c-di-GMP species as a function of time.... With a quantitative model of c-di-GMP oligomerization established that describes the... the buffer conditions and c-di-GMP concentration, and equilibration should be... shown). Kinetic Model of c-di-GMP Self-Association. The experimental results... a simple kinetic model of c-di-GMP oligomerization (Figure 6). As shown above, Figure 6. Kinetic scheme of c-di-GMP oligomer formation. M, monomer; D, dimer; T, tetramer... as a function of total c-di-GMP concentration. (A) Concentrations of the indicated c-di-GMP species (in monomeric units of... as a function of total c-di-GMP concentration in buffer A. concentration of... μM), indeed a significant decrease in c-di-GMP (25 μM) MD intensity (H8, H1... to the canonical H-bonded c-di-GMP dimers, tetramers, or octamers. General... imply a monomeric form of c-di-GMP. Clearly, intracellular stacking complexes with... such heteromeric stacking reactions, c-di-GMP should be monomeric under in... important to note that c-di-GMP adopts a dimeric, self-intercalated form... at low c-di-GMP concentrations. Assessment of c-di-GMP Association from UV... convenient absorption measurement (OD253 < 1), c-di-GMP is usually diluted from millimolar... absorption spectra of 25 μM c-di-GMP after dilution into buffer A. Colors...) species. (B) Population of monomeric c-di-GMP pmono as a function of the... at the low cellular c-di-GMP concentrations. ■ ASSOCIATED CONTENT S Supporting Information...-stacking and G-quartet formation, c-di-GMP can display a rich polymorphism of... presence of metal ions, c-di-GMP exchanges rapidly between monomeric and... The Second Messenger Cyclic Di-GMP; Wolfe, A. J., Visick, K. L., Eds.; John Wiley...